MP0040A

LookOut^® Mycoplasma qPCR Detection Kit

• Synonym(s): Quantiative PCR mycoplasma detection kit
• EC Number: 200-664-3
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• usage: sufficient for 25 reactions
• Quality Level: 200
• quality: Not suitable for clinical diagnostic use.
• packaging: pkg of 1 kit
• storage condition: dry at room temperature
• technique(s): PCR: suitable
• application(s): detection; microbiology
• storage temp.: 2-8°C

Description

General description

The LookOut Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals.

The primer/probe system detects the highly conserved 23S rRNA operon coding region of the mycoplasma genome. The kit is highly specific and does not detect eukaryotic DNA. The detection spectrum includes most mycoplasma species identified as cell culture contaminants (see Table 2 in the technical bulletin). The kit contains the nucleotide dUTP instead of dTTP and is, therefore, suitable for UNG pretreatment.

This kit already contains Taq polymerase in the Mycoplasma Mix.

Application

qPCR Probe Kit for high quality and reliable quantitative detection of mycoplasma DNA in research, industrial application and product testing. Usable for direct testing of cell cultures and biologicals in combination with cell culture enrichment.

Legal Information

LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): 188.6 °F
• Flash Point(C): 87 °C