MBD0049
Escherichia coli FISH probe -ATTO488
Probe for fluorescence in situ hybridization (FISH), 20µM in water
About This Item
Recommended Products
Quality Level
technique(s)
FISH: suitable
fluorescence
λex 504 nm; λem 521 nm (ATTO488)
shipped in
dry ice
storage temp.
−20°C
General description
The FISH technique was successfully used to identify E.coli with the probe in various samples such as pure culture (as described in the figure legends and11,13), large and small intestines samples14-16, fecal samples17-21, colonic biopsies18, urine samples, bladder, and kidney sections embedded in paraffin22 and in E.coli biofilm23. It is strongly recommended to include positive and negative controls in FISH assays to ensure specific binding of the probe of interest and appropriate protocol conditions. We offer positive (MBD0032/33) and negative control (MBD0034/35) probes, that accompany the specific probe of interest.
Escherichia coli is a gram negative, facultative aerobic, rod-shaped coliform bacterium. E. coli colonizes the infant gut within hours of birth and establishes itself as the most abundant facultative anaerobe of the human intestinal microflora for the remainder of life, equipped with the abilities to grow in the ever-changing environment in the gut and cope with the mammalian host interaction.8,9 Nevertheless, E. coli can survive in many different ecological habitats, including abiotic environments, and is considered a highly versatile species. Known habitats of E. coli include soil, water, sediment, and food. Some strains of E. coli have evolved and adapted to a pathogenic lifestyle and can cause different disease pathologies.10
Escherichia coli probe specifically recognizes Escherichia coli cells. Yet some reports describe the recognition of other bacteria with this probe, such as, Shigella boydii, Citrobacter davisae, Citrobacter lapagei, Citrobacter neteri11 and Klebsiella pneumoniae12.
Application
Features and Benefits
- Visualize, identify and isolate Escherichia coli cells.
- Observe native E. coli cell populations in diverse microbiome environments.
- Specific, sensitive and robust identification of E. coli in bacterial mixed population.
- Specific, sensitive and robust identification even when E. coli is in low abundance in the sample.
- FISH can complete PCR based detection methods by avoiding contaminant bacteria detection.
- Provides information on E. coli morphology and allows to study biofilm architecture.
- Identify E. coli in clinical samples such as, urine samples, bladder and kidney sections (formalin-fixed paraffin-embedded (FFPE) samples), fecal samples and colon tissue.
- The ability to detect E. coli in its natural habitat is an essential tool for studying host-microbiome interaction.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
MBD0049-VAR:
MBD0049-50UL-PW:
MBD0049-50UL:
Certificates of Analysis (COA)
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