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MAK539

Sigma-Aldrich

DNA Assay Kit

sufficient for 250 fluorometric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Quality Level

100

detection method

fluorometric

shipped in

ambient

storage temp.

2-8°C

Related Categories

General description

DNA quantitation is a common practice in molecular biology. Very often DNA is available in minute quantities and the traditional UV 260 nm absorbance method requires microgram quantities for reliable results. Accurate determination of DNA concentration, especially when DNA is present at low concentrations, is crucial for reproducible results in sequencing, cloning, transfection and DNA labeling.

Features and Benefits

  • Linear detection range 2 ng to 40 ng (100 – 2,000 ng/mL) calf thymus DNA.
  • Compatible with high-throughput handling systems.
  • Low interference. RNA, salt (up to 3M NaCl), detergent (< 0.01% SDS) and common DNA extraction buffer do not interfere in the assay.

Suitability

The kit is suitable for quantitative determination of DNA in plasmid DNA, genomic DNA, cDNA, DNA following polymerase chain reaction, and DNA extracted from gel and other matrices.

Principle

The DNA Assay Kit is designed to accurately measure nanogram quantities of plasmid DNA, cDNA, DNA following polymerase chain reaction (PCR), and DNA eluted from gels. The improved method utilizes Hoechst dye that binds specifically with double-stranded DNA. The fluorescence intensity, measured at λEx = 350 nm/λEm = 450 nm, is directly proportional to the DNA concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MAK539-1KT:

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