MAK537

D-Amino Acid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Quality Level: 100
• application(s): food and beverages
• detection method: colorimetric; fluorometric
• relevant disease(s): genitourinary disorders
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

D-amino acids are not as widespread as their enantiomeric counterparts in proteins, but they can be found in organisms ranging from bacteria (cell walls and antibiotics) to mammals (central nervous systems). The presence of D-amino acids in food is also of considerable interest. Racemization of L-amino acids during food processing may affect food quality and nutritional value.

Features and Benefits

• Linear detection range: 0.86 to 500 µM for the colorimetric assay and 0.18 to 50 µM for the fluorimetric assay.
• Compatible with high-throughput handling systems.

Suitability

The kit is suitable for D-amino acid determination in tissue, milk, and other biological preparations.

Principle

The D-Amino Acid Assay Kit uses an enzyme-catalyzed oxidation of D-amino acids to convert a dye into a colored and fluorescent form. The absorbance at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the D-amino acid concentration in the sample.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3