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MAK526

Sigma-Aldrich

Diamine Oxidase Assay Kit

sufficient for 100 fluorometric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Quality Level

detection method

fluorometric

relevant disease(s)

cancer; immunological diseases; gastrointestinal diseases

shipped in

wet ice

storage temp.

−20°C

General description

Diamine oxidase (DAO) also known as histaminase or amine oxidase (copper containing), is an enzyme involved in the metabolism, oxidation, and inactivation of histamine in animals. Highest levels are observed in the digestive tract and placenta. An imbalance between histamine intake and the capacity for histamine degradation can lead to histamine intolerance (HIT). Measuring DAO activity in serum can be useful in diagnosing HIT.

Features and Benefits

  • Linear detection range 0.5 to 6 U/L .
  • Compatible with high-throughput handling systems.

Suitability

The kit is suitable for diamine oxidase activity in serum or plasma samples.

Principle

The Diamine Oxidase Assay Kit is based on the oxidation of putrescine to pyrroline plus NH3 and H2O2. The generated H2O2 is then used by HRP to oxidize a dye making it fluorescent. The increase in fluorescence at λEx = 530 nm/λEm = 585 nm is directly proportional to the enzyme activity.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

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PRTR

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FSL

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ISHL Indicated Name

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ISHL Notified Names

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Cartagena Act

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JAN Code

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Certificates of Analysis (COA)

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