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Safety Information

MAK498

Sigma-Aldrich

Alcohol Dehydrogenase (ADH) Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.05

Quality Level

detection method

colorimetric

relevant disease(s)

gastrointestinal diseases

shipped in

wet ice

storage temp.

−20°C

General description

Alcohol dehydrogenase (ADH) is an oxidoreductase which catalyzes the interconversion of alcohols and aldehydes or ketones. ADH is important in humans and other organisms for the break down of alcohols which may otherwise be toxic. In yeast and some bacteria, ADHs catalyze the opposite reaction and produce alcohol as part of fermentation.

Features and Benefits

  • Linear detection range: 0.4 to 80 U/L for 30 minute reaction. Detection Limit of 0.1 U/L for 120 minute reaction.
  • Compatible with high-throughput handling systems.

Suitability

The kit is suitable for alcohol dehydrogenase activity determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)

Principle

The non-radioactive, colorimetric Alcohol Dehydrogenase (ADH) Assay Kit is based on the reduction of the tetrazolium salt MTT in a NADH coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 2


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

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PRTR

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FSL

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ISHL Indicated Name

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ISHL Notified Names

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Cartagena Act

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JAN Code

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Certificates of Analysis (COA)

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