MAK495

Aspartate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• Synonym(s): Aspartate Quantification Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Quality Level: 100
• application(s): pharmaceutical
• detection method: colorimetric; fluorometric
• relevant disease(s): endocrinological disorders, diabetes; neurological disorders
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Aspartate, a nonessential amino acid, is a precursor to several other amino acids and is an excitatory neurotransmitter. Aspartate is involved in the urea cycle and gluconeogenesis.

Features and Benefits

• Linear detection range: 2 to 400 µM aspartate for the colorimetric assay and 1 to 50 µM for the fluorometric assay.

Suitability

The kit is suitable for aspartate determination in plasma, serum, tissue and culture media, as well as for studying the effects of drugs on aspartate metabolism.

Principle

In the Aspartate Assay Kit, aspartate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the aspartate concentration in the sample.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2