MAK443

L-Lactate Assay Kit

Sufficient for 100 fluorometric tests

• Synonym(s): ʟ-Lactic Acid Assay Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Quality Level: 100
• input: cell culture medium; plasma; serum; urine
• detection method: fluorometric
• relevant disease(s): diabetes; pulmonary disorders; endocrinological disorders; cardiovascular diseases; cancer
• storage temp.: −20°C

Description

General description

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology.

Application

The L-Lactate Assay Kit may be used for:

• Lactate Metabolism Research
• Tissue Metabolism Research
• Drug Discovery

Features and Benefits

• Simplified Process: Experience a streamlined process with the addition of only a single working reagent, reducing complexity and saving valuable time and effort.
• Broader Detection Range: Detect L-Lactate levels accurately across a wide range, from 1 to 50 μM, using just 50 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.
• High-Throughput Compatibility: Effortlessly integrate our kit into high-throughput handling systems, ensuring seamless operation and maintaining efficiency and accuracy in large-scale analyses.

Suitability

The kit is suitable for the quantitative determination of L-lactate (L-lactic acid) and the evaluation of drug effects on its metabolism in serum, plasma, urine, cell culture media, and other biological samples.

Principle

The L-Lactate Assay Kit is based on the lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λEx = 530 nm/λEm = 585 nm, is proportional to the lactate concentration in the sample.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids