MAK325

Cholesterol Uptake Assay Kit

sufficient for 100 fluorometric tests

• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 100 fluorometric tests
• detection method: fluorometric
• relevant disease(s): obesity; cardiovascular diseases; cancer
• storage temp.: −20°C

Description

General description

Cholesterol, a crucial sterol and lipid component, is present in cell membranes and circulates in the bloodstream of all animals. It plays essential roles in maintaining cell membrane structure, hormone synthesis, and facilitating cell signaling processes. The intricate regulation of cellular cholesterol levels is paramount, as imbalances have been linked to conditions such as obesity and heart disease. Moreover, heightened cholesterol uptake has been correlated with the rapid proliferation of cancer cells. Through monitoring cellular cholesterol uptake, researchers can delve into these pressing health concerns, advancing both drug discovery efforts and biochemical research aimed at identifying potential treatment modalities.

Application

The Cholesterol Uptake Assay Kit may be used for:

• Drug Discovery
• Cardiovascular Research
• Obesity Research
• Cancer Research

Features and Benefits

• Simplified Process: Experience a streamlined procedure as cell treatment occurs directly in the fluorescence 96-well plate, saving time and effort.
• High-Throughput Compatibility: Seamlessly integrate the kit into high-throughput handling systems for enhanced efficiency and accuracy.

Suitability

Suitable for testing cholesterol uptake by adherent cells, screening of
cholesterol uptake inhibitors, and evaluation of effect of drugs on
cholesterol uptake.

Principle

The cholesterol uptake assay kit is based on cellular uptake of a fluorescently tagged cholesterol probe. The fluorescence intensity measured at λex/em = 485/535 nm is proportional to the amount of cholesterol uptaken by the cells.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Safety Information

• Pictograms: GHS02,GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H225,H319,H336,H371
• Precautionary Statements: P210 - P233 - P240 - P241 - P305 + P351 + P338 - P308 + P311
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 2 - STOT SE 3
• Target Organs: Central nervous system, Eyes,Central nervous system
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1