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MAK310

Sigma-Aldrich

Ammonia Assay Kit

sufficient for 200 fluorometric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

detection method

fluorometric

relevant disease(s)

genitourinary disorders; gastrointestinal diseases

storage temp.

−20°C

Related Categories

General description

Ammonia (NH3) or the ammonium ion (NH4 +) is found in the atmosphere, rainwater, soil, seawater, and volcanic areas. It is widely used as fertilizer, being an important source of nitrogen for living systems. It also plays a role in animal physiology. Ammonia is produced by amino acid metabolism and is converted to urea in the human liver through the urea cycle. Ammonia acts as a precursor for various other nitrogen compounds. It is a colorless gas with pungent odor and can be easily liquefied. It is also used as a feed supplement in livestock industry.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the quantitative determination of ammonia/ammonium concentration in biological samples, such as urine, and environmental samples

Principle

The Ammonia Assay Kit provides a simple and high throughput adaptable assay for quantitative determination of ammonia/ammonium ion concentration in biological samples, such as urine, and environmental samples. This assay is based on the o-phthalaldehyde method in which the reagent reacts with ammonia/ammonium ion producing a fluorometric result ( ex = 360/ em = 450 nm), proportional to the ammonia concentration in the sample. The kit has a linear detection range of 0.012–1 mM ammonia in a 96 well format.

Signal Word

Danger

Hazard Statements

H302 - H314 - H317 - H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

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PRTR

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FSL

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ISHL Indicated Name

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ISHL Notified Names

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Cartagena Act

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JAN Code

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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