MAK203

DPP4 INHIBITOR SCREENING KIT

Sufficient for 100 Fluorometric tests

• Synonym(s): Adenosine Deaminase Complexing Protein-2 Inhibitor Screening Kit, CD26 Inhibitor Screening Kit, Dipeptidyl-Peptidase 4 Inhibitor Screening Kit
• UNSPSC Code: 12161503
• NACRES: NA.25

Properties

• application(s): pharmaceutical
• detection method: fluorometric
• relevant disease(s): endocrinological disorders, diabetes
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... DPP4(1803) ; mouse ... DPP4(13482) ; rat ... DPP4(25253)

Description

General description

Dipeptidyl-peptidase 4 (DPP4; CD26; adenosine deaminase complexing protein-2) is a membrane glycoprotein with serine exopeptidase activity that cleaves X-proline dipeptides from the N-terminus of polypeptides. Inhibitors of DPP4 inhibit the degradation of glucose-dependent insulinotropic polypeptide and glucagon-like peptide-1 by DPP4 and have emerged as oral anti-diabetic agents.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the screening of inhibitors of DPP4.

Principle

The DPP4 Inhibitor Screening Kit is a simple assay suitable for high-throughput screening of potential DPP4 inhibitors. DPP4 activity is measured by cleaving the substrate to yield a fluorescent product (λ_ex = 360/λ_em = 460 nm), proportional to the enzymatic activity present. The effectiveness of the test inhibitors may be compared with the DPP4 inhibitor (sitagliptin) provided as a control.

Kit Components Only

• DPP4 Assay Buffer
• DPP4 Substrate
• DPP4 Enzyme
• DPP4 Inhibitor, Sitagliptin

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup