MAK165

Fluorimetric Hydrogen Peroxide Assay Kit

sufficient for 500 fluorometric tests (red fluorescence)

• Synonym(s): Hydrogen Peroxide Quantification Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 500 fluorometric tests (red fluorescence)
• detection method: fluorometric
• relevant disease(s): cardiovascular diseases; aging/geriatric diseases; orthopedic diseases; pulmonary disorders; neurological disorders
• storage temp.: −20°C

Description

General description

Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

This kit is suitable to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions.

Principle

The Fluorescent Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions. This kit can also be used to detect hydrogen peroxide released from living cells or produced by oxidase activities. This kit utilizes a peroxidase substrate that generates a red fluorescent product (λ_ex = 540/λ_em = 590 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H302,H334
• Precautionary Statements: P261 - P264 - P270 - P284 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable