MAK048

Lipase Activity Assay Kit III

sufficient for 100 fluorometric tests

• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 100 fluorometric tests
• detection method: fluorometric
• storage temp.: −20°C

Description

General description

The lipase family of enzymes catalyzes the cleavage of the ester bonds of lipids. In mammals, this family includes many critical members including pancreatic, hepatic, endothelial, and lipoprotein lipase. Lipases, such as pancreatic lipase, are critical for the metabolism of lipids. Lipases also play key roles in processes such as cell signaling and inflammation. Measurements of lipase activity are commonly used to screen for pancreatic injury or disease, and to monitor diseases such as cystic fibrosis, celiac disease, and Crohn′s disease.

Application

• High-Temperature Short-Time and Holder Pasteurization of Donor Milk: Impact on Milk Composition.: This study evaluates the effects of different pasteurization methods on the composition of donor milk, focusing on its nutritional and biochemical properties (Escuder-Vieco et al., 2021).

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of lipase activity in biological samples including tissue, cells and serum

Principle

The Lipase Activity Assay Kit III provides a simple and direct procedure for measuring lipase activity in a variety of samples. Lipase activity is determined using a coupled enzyme reaction, which results in the generation of methylresorufin (λex = 529/λem = 600 nm) proportional to the enzymatic activity present. One unit of lipase is the amount of enzyme that will generate 1.0 μmole of methylresorufin from the substrate per minute at 37 °C.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids