MAK044

Free Fatty Acid Quantitation Kit

sufficient for 100 colorimetric or fluorometric tests

• Synonym(s): Free Fatty Acid Assay Kit
• UNSPSC Code: 12161503

Properties

• usage: sufficient for 100 colorimetric or fluorometric tests
• application(s): cosmetics; food and beverages
• detection method: colorimetric; fluorometric
• relevant disease(s): endocrinological disorders, diabetes; cancer
• storage temp.: −20°C

Description

General description

Free fatty acids, also know as nonesterified fatty acids, circulate in the plasma bound to albumin. The majority of free fatty acids are derived from either dietary sources or are mobilized from adipose tissue. Plasma free fatty acids are elevated in many obesity-related disorders and may contribute to insulin resistance in peripheral tissues. Conditions such as sepsis and tumors producing lipoactive homones may also be associated with elevated free fatty acid levels.

Abnormalities in fatty acid metabolism causes fatty liver disease. Accumulation of free fatty acid in myocytes leads to the risk of cardiac dysfunction. Increased fatty acid β-oxidation is implicated in cardiac ischemia.

Application

Free Fatty Acid Quantitation Kit has been used in the following:

• to measure lipid hydrolysis, by determining the free fatty acid in digested sample.
• to measure plasma and serum free fatty acids level.
• to quantify free fatty acids level in liver tissue.

Suitability

Suitable for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc.

Principle

In this kit, the concentration of fatty acids (C8 and longer) is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λ_ex = 535/λ_em = 587 nm) product, proportional to the fatty acids present.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup