MAK025

Iron Assay Kit

sufficient for 100 colorimetric tests

• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 100 colorimetric tests
• application(s): cosmetics; food and beverages
• detection method: colorimetric
• relevant disease(s): cancer; hematological disorder
• storage temp.: −20°C

Description

General description

Iron is a mineral that plays an essential role in many biological processes, including DNA synthesis, electron transport and oxygen transport. Iron is a transition element that can form a range of oxidation states, the most common being iron II (Fe²⁺ or ferrous iron) and iron III (Fe³⁺ or ferric iron). Iron-containing proteins participate in many reactions, often utilizing transitory changes in the oxidation state of iron to carry out chemical reactions.

Application

Iron assay kit has been used to determine the concentration of Fe²⁺ in wild type (WT) and Δ ferric uptake regulator (Fur) strains of A. baumannii.

Suitability

Suitable for the measurement of iron concentration in various samples

Principle

In this assay, iron is released by the addition of an acidic buffer. Samples may be tested directly to measure Fe²⁺ or reduced to measure total iron (Fe²⁺ and Fe³⁺). Released iron is reacted with a chromagen resulting in a colorimetric (593 nm) product, proportional to the iron present. The Iron Assay Kit provides a simple convenient means of measuring iron in a variety of biological samples.

Preparation Note

Kit is compatible with serum and tissue samples but not plasma samples.

Safety Information

• Pictograms: GHS05
• Signal Word: Warning
• Hazard Statements: H290,H315,H319
• Precautionary Statements: P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
• Storage Class Code: 8A - Combustible corrosive hazardous materials