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Safety Information

M5814

Sigma-Aldrich

MKK6/SKK3, active human

≥80% (SDS-PAGE), recombinant, expressed in E. coli, Maltose binding protein tagged, buffered aqueous solution

Synonym(s):

MAP Kinase Kinase 6, MAP Kinase Kinase 6/SKK 3

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About This Item

MDL number:
MFCD03457508
UNSPSC Code:
12352200
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

200

Assay

≥80% (SDS-PAGE)

form

buffered aqueous solution

mol wt

81 kDa

UniProt accession no.

P52564

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... MAP2K6(5608)

Specificity

Active in the phosphorylation of p38/SAPK2a

Application

Active enzyme for study of stress-activated protein kinases.
May be used for activation of p38α followed by phosphorylation of MBP.

Unit Definition

1 unit will activate 1 unit of p38/SAPK2 per minute. 1 unit of active p38/SAPK2 will incorporate 1 nanomole of phosphorus into myelin basic protein per minute.

Physical form

Solution in approx. 1 mg/ml in 50 mM Tris-HCl, pH 7.5, 0.1 mM EGTA, 0.1% 2-mercaptoethanol, 0.15 M NaCl, 0.03% Brij® L23, 270 mM sucrose, 0.2 mM PMSF, 1 mM benzamidine.

Legal Information

Brij is a registered trademark of Croda International PLC

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Deleterious substance

JAN Code

M5814-5UG-PW:
M5814-5UG:
M5814-BULK:
M5814-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
087K1648
087K1655
087K1524
024K1492
122K0351

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A Cuenda et al.
The EMBO journal, 16(2), 295-305 (1997-01-15)
Stress-activated protein kinase-3 (SAPK3), a recently described MAP kinase family member with a wide-spread tissue distribution, was transfected into several mammalian cell lines and shown to be activated in response to cellular stresses, interleukin-1 (IL-1) and tumour necrosis factor (TNF)

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