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Safety Information

M5437

Sigma-Aldrich

Anti-MSK-1 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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About This Item

MDL number:
MFCD02686432
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 90 kDa

species reactivity

rat

technique(s)

immunocytochemistry: 1:1,000 using methanol/acetone-fixed rat fibroblast Rat-1 cell line
microarray: suitable
western blot: 1:2,000 using using whole cell lysate of rat fibroblast Rat-1 cell line

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RPS6KA5(9252)
rat ... Rps6ka5(314384)

General description

The mitogen- and stress-activated kinases, MSK-1 and MSK-2 are activated by ERK/MAPK and p38 MAPK in response to growth and cell stress stimuli. MSK-1 and MSK-2 have 75% homology and interact with ERK1/2 and p38 through MAPK-binding domains near the C-terminus. There is a predominant expression of MSK proteins in heart, brain, skeletal muscles, lung, liver, kidney, pancreas and placenta. Highly conserved phosphorylation sites of MSK-1, Ser260 and Thr581, are followed by proline residues indicating that MSK-1 is activated by more than one MAPK. Endogenous MSK-1 is activated in cells by growth factors, phorbol ester stimulation, exposure to UV irradiation, and oxidative or chemical stress and is localized to nucleus of unstimulated or stimulated cells. MSK-1 is directly involved in the regulation of gene expression since it actively phosphorylates, stabilizes and activates many transcription factors. The major transcription factors that are regulated by MSK-1 include CREB, NF-κB and STAT3. MSKs also evidently phosphorylate Histone H3 at Ser10 and Ser28 and HMG at Ser6. Thus a primary role of MSK proteins is also promotion of gene relaxation and activation by regulating the binding of transcription repressors. Anti-MSK-1 specifically recognizes rat MSK-1 (90 kDa).

Immunogen

synthetic peptide corresponding to the C-terminus of human MSK1 (amino acids 783-800). This sequence is not found in the human and mouse MSK2 isoforms.

Application

Anti-MSK-1 may be used at a minimum working dilution of 1:2000 (immunoblotting in whole cell extracts) or 1:1000 (immunocytochemistry) in fibroblast Rat1 cell line. The antobody has also been used for detection by immunoblotting in mouse fibroblast 10T1/2 cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

M5437-.2ML:
M5437-VAR:
M5437-BULK:
IXO11233:

Certificates of Analysis (COA)

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Linda Vermeulen et al.
Trends in biochemical sciences, 34(6), 311-318 (2009-05-26)
Among the mitogen-activated protein kinase (MAPK) targets, MSKs (mitogen- and stress-activated protein kinases) comprise a particularly interesting protein family. Because MSKs can be activated by both extracellular-signal-regulated kinase and p38 MAPKs, they are activated by many physiological and pathological stimuli.
Y Zhang et al.
The Journal of biological chemistry, 276(45), 42534-42542 (2001-09-13)
Phosphorylation of Tyr(705) and Ser(727) of signal transducer and activator of transcription 3 (STAT3) are known to be required for maximal activation by diverse stimuli. Tyr(705) phosphorylation is generally accepted to be mediated by the Janus kinase family. But the
S Thomson et al.
The EMBO journal, 18(17), 4779-4793 (1999-09-02)
The nucleosomal response refers to the rapid phosphorylation of histone H3 on serine 10 and HMG-14 on serine 6 that occurs concomitantly with immediate-early (IE) gene induction in response to a wide variety of stimuli. Using antibodies against the phosphorylated
Marie Cargnello et al.
Microbiology and molecular biology reviews : MMBR, 75(1), 50-83 (2011-03-05)
The mitogen-activated protein kinases (MAPKs) regulate diverse cellular programs by relaying extracellular signals to intracellular responses. In mammals, there are more than a dozen MAPK enzymes that coordinately regulate cell proliferation, differentiation, motility, and survival. The best known are the
Linda Vermeulen et al.
The EMBO journal, 22(6), 1313-1324 (2003-03-12)
Nuclear factor kappaB (NF-kappaB) is one of the key regulators of transcription of a variety of genes involved in immune and inflammatory responses. NF-kappaB activity has long been thought to be regulated mainly by IkappaB family members, which keep the
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