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M4439

Sigma-Aldrich

Anti-c-Myc antibody, Mouse monoclonal

clone 9E10, purified from hybridoma cell culture

Synonym(s):

Anti-c-Myc

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.56

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

9E10, monoclonal

form

buffered aqueous solution

mol wt

62 kDa

species reactivity

human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:5000 using c-myc tagged fusion protein

UniProt accession no.

P01106

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... MYC(4609)

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General description

The mouse Anti-c-MYC monoclonal antibody recognizes an epitope located within a sequence (EQKLISEEDL) of the product of the human oncogene c-myc.
c-Myc (v-myc avian myelocytomatosis viral oncogene homolog) is a transcription factor, which is coded by MYC gene. It was first described as a homolog of an avian retroviral oncogene. It belongs to the family of transcription factors which are characterized by basic region helix-loop-helix/leucine zipper. Along with N-Myc and L-Myc, it forms the MYC proto-oncogene family. It forms a heterodimer with MAX, and this heterodimer binds to the E-boxes/CACGTG sequence motif on DNA. This gene is localized to human chromosome 8q24.

Immunogen

synthetic peptide of the human p62c-Myc protein.

Application

Monoclonal Anti-c-Myc antibody produced in mouse is suitable for immunoprecipitation, immunoblotting, ELISA, immunofluorescence, microarray and electron microscopy. It has also been used in ChIP (Chromatin Immunoprecipitation).

Biochem/physiol Actions

v-myc avian myelocytomatosis viral oncogene homolog belongs to MYC family. When overexpressed these proteins can cause DNA double-stand breaks (DSBs) and genome instability. This transcription factor MYC helps in regulating long noncoding RNAs (lncRNAs) which has been implicated in cancer cell proliferation and tumorigenesis. Its overexpression is also associated with various cancers, and in particular B cell lymphomas. Increased expression of c-MYC has a negative effect on the ability of B cell lymphomas to functionally present Ags/peptides to CD4+ T cells. This protein exerts suppressive effects at several critical checkpoints in Ag presentation. It is associated with Burkitt lymphoma and a group of diffuse large B-cell lymphoma (DLBCL).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Working dilution: 1:5000

Other Notes

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

M4439-BULK:
M4439-100UL:
M4439-VAR:

Certificates of Analysis (COA)

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Željka Škunca et al.
Acta medica Croatica : casopis Hravatske akademije medicinskih znanosti, 68(3), 299-305 (2015-05-29)
Diffuse large B-cell lymphoma (DLBCL) is classified as lymphoma and various entities using the gene expression of proteins are classified into three groups. The aim of this study was to clarify the clinical, biological, immunophenotypic and cytogenetic features of DLBCL
Taewan Kim et al.
Journal of the National Cancer Institute, 107(4), doi:10-doi:10 (2015-02-11)
The functions of long noncoding RNAs (lncRNAs) have been identified in several cancers, but the roles of lncRNAs in colorectal cancer (CRC) are less well understood. The transcription factor MYC is known to regulate lncRNAs and has been implicated in
Susanna Ambrosio et al.
Mutation research, 774, 6-13 (2015-03-17)
Although it is established that when overexpressed, the MYC family proteins can cause DNA double-stand breaks (DSBs) and genome instability, the mechanisms involved remain unclear. MYC induced genetic instability may result from increased DNA damage and/or reduced DNA repair. Here
Mirjana Macvanin et al.
Journal of bacteriology, 194(22), 6046-6055 (2012-09-04)
Some unidentified RNA molecules, together with the nucleoid protein HU, were suggested to be involved in the nucleoid structure of Escherichia coli. HU is a conserved protein known for its role in binding to DNA and maintaining negative supercoils in
Jun-Sub Kim et al.
Molecular biology of the cell, 20(11), 2650-2660 (2009-04-03)
Cellular stimuli generate reactive oxygen species (ROS) via the local action of NADPH oxidases (Nox) to modulate cytoskeletal organization and cell migration through unknown mechanisms. Cofilin is a major regulator of cellular actin dynamics whose activity is controlled by phosphorylation/dephosphorylation
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