L7395

Lipoxidase from Glycine max (soybean)

Type I-B, lyophilized powder, ≥50,000 units/mg solid

• Synonym(s): 15-lipoxygenase, LOX A, Linoleate:oxygen oxidoreductase, Lipoxygenase
• CAS Number: 9029-60-1
• Enzyme Commission number: 1.13.11.12 (BRENDA, IUBMB)
• EC Number: 232-853-1
• MDL number: MFCD00131527
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Type I-B
• form: lyophilized powder
• specific activity: ≥50,000 units/mg solid
• mol wt: 94 kDa
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Lipoxidases (LOXs), or lipoxygenases, are nonheme iron proteins. Lipoxygenases are widely present in plants, some microorganisms, and animals. They are categorized as LOX-1, LOX-2, and LOX-3 with respect to pH and optimum temperature, substrate specificity, and level of inhibition by different agents.

Application

Lipoxidase from Glycine max (soybean) has been used:

• to study the effects of 2-(α-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol(TMG) on oxidation of lipoproteins
• as a representative enzyme for lipoxygenases in microplate assay platform to screen for enzymes degrading wood extractives
• as a positive control in lipoxygenase inhibitory activity assay

The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).

Biochem/physiol Actions

Lipoxidases are deactivated by heat treatment near 100°C for 5 to 10 minutes. Studies show that gamma irradiation treatment decreases the specific activity of soybean lipoxygenases. These enzymes are used in studies related to asthma and allergies. Lipoxidases are applicable for industrial bio-transformations such as in the production of aroma fine chemicals.

Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Unit Definition

One unit will cause an increase in A₂₃₄ of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A₂₃₄ unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.

Physical form

Contains stabilizer and NaCl

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves