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Safety Information

L2140

Sigma-Aldrich

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

Isolectin B4 (BSI-B4), biotin conjugate, lyophilized powder

Synonym(s):

Bandeirea simplicifolia agglutinin, BS-I

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

Bandieraea simplicifolia

Quality Level

200

conjugate

biotin conjugate

form

lyophilized powder

potency

<50 μg per mL agglutination activity (using human blood group B erythrocytes)

composition

Protein, ~95% E1%/280

extent of labeling

3-6 mol biotin per mol protein

storage temp.

−20°C

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General description

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has A and B subunits, which recognizes αGalNAc and αGal-end groups respectively. Lectins are extracted from the seeds of the legume shrub Griffonia simplicifolia, which are carbohydrate binding proteins.

Application

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has been used for immunohistochemistry and immunofluorescence staining.

Biochem/physiol Actions

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.
Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) agglutinates human blood group A and B cells. It is specific for αGalNAc- and αGal. It acts as a reagent for the detection of this αGal epitope in biological materials. Lectin from Bandeiraea simplicifolia is used as a marker for xenoreactive antigen (αGal1−3Gal) and cancer. Lectin plays an important role in fertilization, embryogenesis, inflammation, metastasis and host-parasite recognition.

Other Notes

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Physical form

Contains citrate buffer salts and calcium chloride

Analysis Note

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

L2140-.2MG:
L2140-BULK:
L2140-VAR:
L2140-1MG-PW:
L2140-1MG:
L2140-.2MG-PW:

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Certificates of Analysis (COA)

Lot/Batch Number
039M4066V
109M4097V
11180916
058M4101V
11171215

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Sensory processing of deep tissue nociception in the rat spinal cord and thalamic ventrobasal complex
Sikandar S, et al.
Physiological Reports, 5(14), e13323-e13323 (2017)
Isolectins IA and IB of Griffonia (Bandeiraea) simplicifolia crystal structure of metal-free gs i-b4 and molecular basis for metal binding and monosaccharide specificity
Lescar J, et al.
The Journal of Biological Chemistry, 277(8), 6608-6614 (2002)
Quan Yuan et al.
Tissue engineering. Part A, 24(9-10), 719-728 (2017-10-06)
The aim of this study was to analyze the three-dimensional distribution of hypoxia in the arteriovenous (AV) loop model in rats, by examining the distribution of hypoxia-inducible factor-1 alpha (HIF-1α). AV loops were created from the femoral artery and vein
Ludovic Wrobel et al.
Neuroscience letters, 495(1), 49-54 (2011-03-23)
Oxytocin can influence various spinal functions. However, little is known about the spinal neuronal networks responsible for oxytocin effects. The aim of this study was to localize and characterize spinal neurons expressing oxytocin receptors. We used an oxytocin receptor-reporter mouse
The Lectins: Properties, Functions, and Applications in Biology and Medicine (2012)
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