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Safety Information

L1900

Sigma-Aldrich

Luria broth base, Miller′s modified

Tryptone, yeast extract and sodium chloride

Synonym(s):

Lennox broth base, modified, Luria-Bertani broth base, modified, Lysogeny broth base, modified

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About This Item

UNSPSC Code:
41106200
NACRES:
NA.72

form

powder

technique(s)

microbiological culture: suitable

application(s)

agriculture
microbiology

General description

Luria broth (LB) is a nutritionally rich bacterial culture medium that is commonly used for maintaining and cultivating laboratory recombinant strains of E. coli. It has potential applications in molecular microbiology methods such as the preparation of plasmid DNA and recombinant proteins. Luria broth base, Miller′s modified is used to culture members of the family Enterobacteriaceae and for coliphage plaque assays.

Application

Luria broth base, Miller′s modified has been used as a nutrient culture medium for E-coli cells.

Luria broth base (LB), Miller (L1900) is a bacteria growth mixture of sodium chloride (0.5g/L); tryptone (10g/L) and yeast extract (5g/L) typically combined with agar (15g/L).

Components

Contains: Tryptone, yeast extract and sodium chloride.

Reconstitution

Recommended use at 15.5 g per liter.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

L1900-BULK:
L1900-250G:
L1900-1KG:
L1900-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Erik Benson et al.
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It was suggested more than thirty years ago that Watson-Crick base pairing might be used for the rational design of nanometre-scale structures from nucleic acids. Since then, and especially since the introduction of the origami technique, DNA nanotechnology has enabled
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Applied and environmental microbiology, 70(11), 6435-6443 (2004-11-06)
We systematically investigated the physiological response as well as DNA damage repair and damage tolerance in Shewanella oneidensis MR-1 following UVC, UVB, UVA, and solar light exposure. MR-1 showed the highest UVC sensitivity among Shewanella strains examined, with D37 and
C Niu et al.
Applied and environmental microbiology, 70(12), 6951-6956 (2004-12-03)
The specific biofilm formation (SBF) assay, a technique based on crystal violet staining, was developed to locate plant essential oils and their components that affect biofilm formation. SBF analysis determined that cinnamon, cassia, and citronella oils differentially affected growth-normalized biofilm
Anthony S Danko et al.
Applied and environmental microbiology, 70(10), 6092-6097 (2004-10-07)
Pseudomonas putida strain AJ and Ochrobactrum strain TD were isolated from hazardous waste sites based on their ability to use vinyl chloride (VC) as the sole source of carbon and energy under aerobic conditions. Strains AJ and TD also use
Daria Julkowska et al.
Journal of bacteriology, 187(1), 65-76 (2004-12-17)
The natural wild-type Bacillus subtilis strain 3610 swarms rapidly on the synthetic B medium in symmetrical concentric waves of branched dendritic patterns. In a comparison of the behavior of the laboratory strain 168 (trp) on different media with that of
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