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Safety Information

L1044

Sigma-Aldrich

Anti-Leukemia Inhibitory Factor antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym(s):

CDF, D factor, DIA, Emfilermin, LIF, differentiation inhibitory activity

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About This Item

MDL number:
MFCD00283185
UNSPSC Code:
51111800
NACRES:
NA.41

biological source

goat

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

mouse

technique(s)

neutralization: suitable
western blot: 0.1-0.2 μg/mL

UniProt accession no.

P09056

storage temp.

−20°C

Gene Information

mouse ... Lif(16878)

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General description

Leukemia inhibitory factor (LIF) is a pleotropic glycoprotein that belongs to the IL6 family of cytokines. It is secreted by many cell types such as, fibroblasts, macrophages, T cells, monocytes, liver cells, osteoblasts and cells of endometrium. LIF interacts with a heterodimeric receptor composed of two subunits, gp130 and gp190. A variety of signal transduction pathways are triggered in response to LIF including ERK/MAPK, JAK1, PI3K, STAT3, Jun-cFos and NF-κB. The various functions of LIF include proliferation of adult human T cells, maintenance of pluripotency of embryonic stem cells and inhibition of leukemic ell differentiation. Reports also indicate the important role of LIF in lactation, tissue regeneration after brain and spinal cord injury, muscle regeneration and motor neuron functions.
Anti-Leukemia Inhibitory Factor recognizes mouse leukemia inhibitory factor. It shows less than 5% cross-reactivity with recombinant human LIF.

Immunogen

purified, E. coli-derived recombinant mouse leukemia inhibitory factor.

Application

Anti-Leukemia Inhibitory Factor may be used in neutralization reactions at a working concentration of 0.5 ng/ml. For immunoblotting a concentration of 0.1-0.2 μg/ml is recommended.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

L1044-100UG:
L1044-VAR:
L1044-100UG-PW:
L1044-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ekaterini A Kritikou et al.
Development (Cambridge, England), 130(15), 3459-3468 (2003-06-18)
STAT3 is the key mediator of apoptosis in mammary gland. We demonstrate here that LIF is the physiological activator of STAT3, because in involuting mammary glands of Lif(-/-) mice, pSTAT3 is absent and the STAT3 target, C/EBPdelta, is not upregulated.
Sylvian Bauer et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 26(46), 12089-12099 (2006-11-17)
Although neural stem cells (NSCs) persist in various areas of the adult brain, their contribution to brain repair after injury is very limited. Treatment with exogenous growth factors can mitigate this limitation, suggesting that the brain environment is normally deficient
L C Hunt et al.
Experimental cell research, 316(6), 1002-1009 (2009-12-08)
Leukemia inhibitory factor (LIF) is an important regulator of skeletal muscle regeneration and has been suggested to be mitogenic for myogenic cells because it has been shown to increase the quantity of myoblast cells grown in culture over extended periods
Marie-Emmanuelle Mathieu et al.
Stem cell reviews, 8(1), 1-15 (2011-05-04)
LIF, a member of the IL6 family of cytokine, displays pleiotropic effects on various cell types and organs. Its critical role in stem cell models (e.g.: murine ES, human mesenchymal cells) and its essential non redundant function during the implantation
D J Hilton
Trends in biochemical sciences, 17(2), 72-76 (1992-02-01)
Leukaemia inhibitory factor (LIF) is one of a growing number of cytokines that cannot be readily categorized according to its functions. Rather, these pleiotropic hormones have diverse and often overlapping effects on a multitude of cell types: for example, LIF
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