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Safety Information

I7526

Sigma-Aldrich

Anti-Interleukin-4 antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym(s):

Anti-IL-4

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About This Item

MDL number:
MFCD00212213
UNSPSC Code:
51111800
NACRES:
NA.41

biological source

goat

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

human

technique(s)

indirect ELISA: suitable
neutralization: suitable
western blot: suitable

UniProt accession no.

P05112

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... IL4(3565)

General description

Interleukin-4 (IL-4) is a T cell derived chemokine that stimulates the Th2 mediated immune response and proliferation of B cells. The effects of IL-4 are mediated by two types of receptors, type I receptor consisting of IL-4Rα and common γ chain and type II receptor composed of IL-4Rα and IL-13Rα1. The signaling stimulated by IL-4 leads to activation of JAK/STAT6 and IRS-mediated PI3K/Akt pathway. Through these pathways, IL-4 is responsible for endocytic activity of macrophages, chemotaxis of leukocytes in response to inflammation, angiogenesis and regulation of nitric oxide metabolism in macrophages. Anti-tumor effects of IL-4 have been reported in cancers of breast, liver and renal cells. Anti-Interleukin-4 specifically recognizes human interleukin-4. The antibody shows no cross reactivity with rmIL-4 and rrIL-4.

Specificity

The antibody will neutralize the biological activity of recombinant human IL-4, but not recombinant mouse IL-4.

Immunogen

recombinant human IL-4.

Application

Anti-Interleukin-4 antibody may be used for immunoblotting at a working concentration of 0.1-0.2 μg/ml. For ELISA, a working concentration of 0.5-1.0 μg/ml is recommended. The antibody may be used for immunohistochemistry of cultured cells or tissue sections at a working antibody concentration of 0.5-5 μg/ml. The antibody is suitable for neutralization assay; ND50 is 0.02-0.1 μg/ml. Anti-IL-4 antibody was used for immunohistochemistry and ELISA using cervicovaginal tissue sections and cervicovaginal extracts of Belgium white rabbits.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Physical form

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

I7526-VAR:
I7526-BULK:
I7526-.1MG:
I7526-.1MG-PW:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Toll-like receptors and cytokines as surrogate biomarkers for evaluating vaginal immune response following microbicide administration
Gupta SM et al
Mediators of Inflammation, doi: 10-doi: 10 (2008)
Investigational therapeutics targeting the IL-4/IL-13/STAT-6 pathway for the treatment of asthma
Oh CK et al
European Respiratory Review, 19, 11546-11554 (2010)
M Stein et al.
The Journal of experimental medicine, 176(1), 287-292 (1992-07-01)
Expression of the macrophage mannose receptor is inhibited by interferon gamma (IFN-gamma), a T helper type 1 (Th-1)-derived lymphokine. Interleukin 4 (IL-4), a Th-2 lymphocyte product, upregulates major histocompatibility class II antigen expression but inhibits inflammatory cytokine production by macrophages.
Alberto Mantovani et al.
Trends in immunology, 25(12), 677-686 (2004-11-09)
Plasticity and functional polarization are hallmarks of the mononuclear phagocyte system. Here we review emerging key properties of different forms of macrophage activation and polarization (M1, M2a, M2b, M2c), which represent extremes of a continuum. In particular, recent evidence suggests
E M Coccia et al.
International immunology, 12(7), 977-985 (2000-07-06)
NO is a labile radical involved in several immunological, antimicrobial and inflammatory processes. In macrophages, NO formation is catalyzed by the cytokine-inducible enzyme inducible NO synthase (iNOS). The importance of IFN regulatory factor (IRF)-1 and of the signal transducers and
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