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Safety Information

HT101128

Sigma-Aldrich

Bouin′s solution

histological fixative

Synonym(s):

Bouin’s fluid

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About This Item

MDL number:
MFCD00146169
UNSPSC Code:
12171500
NACRES:
NA.47

shelf life

Expiry date on the label

composition

acetic acid, 5%
formaldehyde, 9%
picric acid, 0.9%

color

yellow

application(s)

hematology
histology

storage temp.

room temp

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General description

Bouin′s solution is a noncoagulant yellow stain fixative. It imposes shrinkage on tissues when used as preservative due to its picric acid component. It is majorly used in ureteroscopic biopsies. It preserves morphological and nuclear information in tissues upon fixation.

Application

Bouin′s solution has been used:
  • in the staining of human liver biopsy tissue samples for two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) imaging
  • in the fixation of rat ovaries for the observing follicle cells using microscopy
  • in the fixation of gonads of crab for morphological and histological analysis

Prepared with saturated picric acid, formaldehyde and acetic acid. Excellent fixative for preserving soft and delicate structures. Used as a mordant in various trichrome procedures.

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Deleterious substance

PRTR

Specified Class I Designated Chemical Substances

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

HT101128-4L:4548173286778
HT101128-VAR:
HT101128-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nonlinear optical microscopy: use of second harmonic generation and two-photon microscopy for automated quantitative liver fibrosis studies
Sun W, et al.
Journal of Biomedical Optics, 13(6), 064010-064010 (2008)
Improving the interpretation of ureteroscopic biopsies: use of Bouin's fixative
Bultitude MF, et al.
BJU International, 108(9), 1373-1375 (2011)
DNA and RNA obtained from Bouin?s fixed tissues
Bonin S, et al.
Journal of Clinical Pathology, 58(3), 313-316 (2005)
Testis Maturation Stages of Mud Crab (Scylla olivacea) Broodstock on Different Diets
Ikhwanuddin M, et al.
Sains Malaysiana, 47(3), 427-432 (2018)
Angelica B Bernal et al.
PloS one, 5(12), e15558-e15558 (2010-12-24)
We have shown recently that maternal undernutrition (UN) advanced female pubertal onset in a manner that is dependent upon the timing of UN. The long-term consequence of this accelerated puberty on ovarian function is unknown. Recent findings suggest that oxidative
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