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HPA009026

Sigma-Aldrich

Anti-RRBP1 antibody produced in rabbit

enhanced validation

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-180 kDa ribosome receptor homolog antibody produced in rabbit, Anti-ES/130-related protein antibody produced in rabbit, Anti-Ribosome receptor protein antibody produced in rabbit, Anti-Ribosome-binding protein 1 antibody produced in rabbit

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About This Item

UNSPSC Code:
12352203
Human Protein Atlas Number:
HPA009026
NACRES:
NA.43

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human

enhanced validation

orthogonal RNAseq
independent
Learn more about Antibody Enhanced Validation

technique(s)

immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:2500-1:5000

immunogen sequence

ELHSKLQSSEAEVRSKCEELSGLHGQLQEARAENSQLTERIRSIEALLEAGQARDAQDVQASQAEADQQQTRLKELESQVSGLEKEAIELREAVEQQKVKNNDLREK

UniProt accession no.

Q9P2E9

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RRBP1(6238)

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General description

RRBP1 (ribosome binding protein 1) is a membrane protein that localizes to endoplasmic reticulum (ER). It is also found in cytoplasm and nucleus. This protein is composed of 1410 amino acids, a transmembrane domain in its hydrophobic N-terminal, a highly conserved tandem repeat which functions as the ribosome-binding domain, and the C-terminal which contains an acidic coiled-coil domain.

Immunogen

Ribosome-binding protein 1 recombinant protein epitope signature tag (PrEST)

Application

Anti-RRBP1 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

Biochem/physiol Actions

RRBP1 (ribosome binding protein 1) is a key ribosome binding protein and plays essential role in the translocation of naïve proteins across the membrane of endoplasmic reticulum (ER). It is essential for the terminal differentiation of secretory tissues, and for the production of pro-collagen in secretory tissues. It also functions as the interacting partner of the motor protein KIF5B (kinesin family member 5B), which is up-regulated in several cancer cell lines. RRBP1 protein is highly expressed in breast cancer (BC) cells and might have potential as a BC marker. This protein is essential for the ER-localization of bulk poly(A) and of certain mRNAs such as, placental alkaline phosphatase and calreticulin. Up-regulation of this protein promotes the progression of colorectal cancer, and functions as a predictor of poor outcome in post-operative cases. In secretory cells, RRBP1-rich ER is responsible for the expansion of trans-Golgi network following the secretion of activated collagen.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST71890

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

HPA009026-100UL:
HPA009026-25UL:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Payam Benyamini et al.
Molecular biology of the cell, 20(2), 732-744 (2008-11-28)
We have previously reported that the expression in yeast of an integral membrane protein (p180) of the endoplasmic reticulum (ER), isolated for its ability to mediate ribosome binding, is capable of inducing new membrane biogenesis and an increase in secretory
Deepthi Telikicherla et al.
Clinical proteomics, 9(1), 7-7 (2012-06-20)
The molecular events that lead to malignant transformation and subsequent metastasis of breast carcinoma include alterations in the cells at genome, transcriptome and proteome levels. In this study, we used publicly available gene expression databases to identify those candidate genes
C T Basson et al.
Genomics, 35(3), 628-631 (1996-08-01)
The chicken extracellular matrix glycoprotein ES/ 130 is necessary for epithelial--mesenchymal transformation in the developing heart and is also expressed in noncardiac chicken tissues such as limb and notochord. We have identified hES, the human homolog of chicken ES/130. Fluorescence
Kiyoko Ogawa-Goto et al.
Molecular biology of the cell, 18(10), 3741-3751 (2007-07-20)
p180 was originally reported as a ribosome-binding protein on the rough endoplasmic reticulum membrane, although its precise role in animal cells has not yet been elucidated. Here, we characterized a new function of human p180 as a microtubule-binding and -modulating
Tomonori Ueno et al.
Experimental cell research, 316(3), 329-340 (2009-11-26)
A coiled-coil endoplasmic reticulum (ER) protein, p180, was originally reported as a ribosome-binding receptor on the rough ER and is highly expressed in secretory tissues. Recently, we reported new functions of p180 as a microtubule-bundling protein on the ER. Here
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