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H9034

Sigma-Aldrich

Hepatitis C virus core antigen

~1 mg/mL, ≥95% (SDS-PAGE), recombinant, expressed in E. coli

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About This Item

MDL number:
MFCD02264926
UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

200

Assay

≥95% (SDS-PAGE)

mol wt

22 kDa (plus 114 kDa galactosidse)

concentration

~1 mg/mL

UniProt accession no.

G9G988
L7S4V3

shipped in

dry ice

storage temp.

−20°C

Gene Information

hepatitis C virus ... HCVgp1(951475)
human ... HBVgp4(944568)

General description

Hepatitis C virus core antigen (HCV-cAg) is a well-preserved antigenic protein of the internal capsid.
Hepatitis C virus glycoprotein 1 (HCVgp1), also referred to as polyprotein, is crucial for the induction of double-membrane vesicles, sites of HCV RNA amplification. The amino terminal of polyprotein contains the structural proteins core, envelope protein 1 (E1) and E2. The proteolytic cleavage of polyprotein results in the formation of 10 mature viral proteins which are needed for viral replication and assembly.
amino acids 2-192 of HCV polyprotein

Specificity

Reacts strongly with human HCV positive serum.

Application

Hepatitis core antigen (HCA) has been used as an analyte in enzyme-linked immunosorbent assay (ELISA) to study the mechanism of bovine serum albumin (BSA) blocking in common ELISA protocols.

Biochem/physiol Actions

Hepatitis C virus core antigen (HCV-cAg) may be a less expensive way to diagnose active hepatitis C virus (HCV) infection in the hemodialysis (HD) population.
Positive control for HCV antibodies.

Other Notes

HCV core antigen [2-192]-Galactosidase-tagged at the N-terminus.

Physical form

Solution in 8 M Urea, 20 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Deleterious substance

JAN Code

H9034-100UG:
H9034-BULK:
H9034-100UG-PW:
H9034-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCG6190
SLCG4357
SLCF6015
SLBW9151
SLBR0762V

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Margaret A Scull et al.
PLoS pathogens, 11(11), e1005297-e1005297 (2015-11-21)
The hepatitis C virus (HCV) p7 protein is required for infectious virus production via its role in assembly and ion channel activity. Although NMR structures of p7 have been reported, the location of secondary structural elements and orientation of the
Inés Romero-Brey et al.
mBio, 6(4), e00759-e00759 (2015-07-15)
Induction of membrane rearrangements in the cytoplasm of infected cells is a hallmark of positive-strand RNA viruses. These altered membranes serve as scaffolds for the assembly of viral replication factories (RFs). We have recently shown that hepatitis C virus (HCV)

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