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Safety Information

H5042

Sigma-Aldrich

Cytochrome P450 3A4 human

recombinant, expressed in E. coli

Synonym(s):

Cytochrome P450, purified, soluble, 1,4-cineole 2-exo-monooxygenase, Albendazole sulfoxidase, CYP3A3, CYP3A4, Cytochrome P450 NF-25, Cytochrome P450-PCN1

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About This Item

CAS Number:
9035-51-2
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

human

Quality Level

200

recombinant

expressed in E. coli

description

purified, soluble

Assay

3.5—25 mg protein/mL (Bradford)

form

liquid

specific activity

12-200 units/mg protein

mol wt

57318.7 g/mol

concentration

3.5-25 mg protein/mL (protein)
50-150 nmol/mL (P450)
6-14 nmol/mg protein (specific content)

technique(s)

cell based assay: suitable

solubility

water: soluble

NCBI accession no.

NP_059488.2

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CYP3A4(1576)

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General description

Research area: IMMUNO AND CKS

Cytochrome P450 3A4 (CYP3A4) indicates a mammalian enzyme (CYP) family 3, subfamily A, gene 4. There are 57 CYP genes and 33 pseudogenes in humans, organized into 18 families and 42 subfamilies. Among these, the 3A4 isoform (CYP3A4) is the most prevalent and is primarily found in the liver and gastrointestinal tract.

Application

Cytochrome P450 3A4 human has been used in circular dichroism analysis.

Biochem/physiol Actions

Cytochrome P450 3A4 (CYP3A4) is one of the isoforms of cytochrome P450 (P450s). Cytochrome P450s (P450s) plays a vital role in processing endogenous compounds and xenobiotics, including pollutants, environmental compounds, and drug molecules. CYP3A4 oxidizes more than 90% of marketed drugs through various metabolic processes.CYP3A4 mRNA expression is upregulated in pediatric Crohn’s disease. Moreover, it provides high tolerance against herbicides such as mefenacet, amiprofos-methyl, pyributicarb, norflurazon, trifluralin, pendimethalin, chlorotoluron, and chloroacetamides.

Unit Definition

One unit will convert 1.0 nanomole of testosterone to 6-beta-testosterone per minute at pH 7.4 at 37 °C.

Other Notes

Supplied as purified soluble proteins, rather than microsomal preparations.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

H5042-1000PMO:
H5042-1000PMO-PW:
H5042-1000PMO-LBL:
H5042-BULK:
H5042-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
SLBZ7288
SLBZ7290
SLBV1549
SLBR6840V
SLBP1569V

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Modulation of Carbon Nanotubes' Perturbation to the Metabolic Activity of CYP3A4 in the Liver
Zhang Y, et al.
Advances in Functional Materials, 26(6), 841-850 (2016)
Pharmacokinetic-pharmacodynamic consequences and clinical relevance of cytochrome P450 3A4 inhibition
Dresser, et al.
Clinical Pharmacokinetics, 38(1), 41-57 (2000)
Crystal structures of human cytochrome P450 3A4 bound to metyrapone and progesterone
Williams PA, et al.
Science, 305(5684), 683-686 (2004)
Yicong Bian et al.
Drug metabolism and disposition: the biological fate of chemicals, 43(9), 1336-1340 (2015-06-14)
Minipigs represent a good animal model because of the physiologic and anatomic similarities they share with humans. Three cytochrome P450 (CYP) 3A isozymes, CYP3A22, CYP3A29, and CYP3A46, have recently been reported to be expressed in Bama minipigs, which have limited
Haifeng Zhang et al.
PloS one, 10(6), e0128547-e0128547 (2015-06-06)
Human cytochrome b5 (Cyt b5) plays important roles in cytochrome P450 (CYP)-mediated drug metabolism. However, the expression level of Cyt b5 in normal human liver remains largely unknown. The effect of Cyt b5 on overall CYP activity in human liver
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