H3791

Anti-HOXA3 antibody produced in rabbit

~1.0 mL/mL, affinity isolated antibody

• Synonym(s): Anti-HOX1, Anti-HOX1E, Anti-Homeobox A3, Anti-MGC10155
• MDL number: MFCD02263083
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~60 kDa
• species reactivity: human, mouse
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mL/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells expressing human HOXA3; indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human HOXA3; western blot: 2-4 μg/mL using lysates of HEK-293T cells over expressing human HOXA3
• UniProt accession no.: O43365
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... HOXA3(3200) ; mouse ... Hoxa3(15400)

Description

General description

Homeobox A3 (HOXA3) transcription factor/protein belongs to the HOX family. The HOXA3 gene is mapped on the human chromosome at 7p15.2.

Specificity

Anti-HOXA3 specifically recognizes HOXA3.

Application

Anti-HOXA3 antibody produced in rabbit may be used in:

• proximity ligation assay
• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

Disruption of the homeobox A3 (HOXA3) gene results in embryonic lethality, which was rescued by placing HOXD3. HOXA3 gene expression is upregulated during neovascularization as a result of injury indicating that HOXA3 promotes angiogenesis. Mutations of the HOXA3 gene are associated with defects in thymus organogenesis and parathyroid development.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Storage and Stability

Store at −20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze at −20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Discard working dilutions if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable