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H0892

Sigma-Aldrich

Anti-Hemocyanin (KLH) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
MFCD00162375
UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

keyhole limpet

packaging

antibody small pack of 25 μL

technique(s)

indirect ELISA: 1:20,000

shipped in

ambient

storage temp.

−20°C

target post-translational modification

unmodified

General description

Hemocyanin is a large, multi-subunit respiratory protein present in arthropods and mollucs. They circulate extracellularly in hemolymph. Hemocyanins do not contain iron but bind oxygen at an active site containing copper atoms ligated by protein side chains.

Application

Anti-Hemocyanin (KLH) antibody produced in rabbit has been used in:
  • immunocytochemistry{58
  • }immunohistochemistry
  • immunofluorescent staining
  • immunoblotting

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)

Biochem/physiol Actions

Keyhole limpet hemocyanin has been used traditionally as a protein carrier for hapten-carrier conjugates in immunochemistry. Keyhole limpet hemocyanin (KLH) has been regarded as a superior carrier. Affinity isolated antibody to hemocyanin may be used as a control reagent for the preparation and immunization of hapten-carrier conjugates.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

H0892-BULK:
H0892-1ML:
H0892-25UL:
H0892-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Antonio Castro et al.
Journal of chemical neuroanatomy, 35(4), 306-316 (2008-05-09)
Immunocytochemical techniques were used to investigate the appearance and distribution of calretinin in the olfactory system of developing and adult brown trout (Salmo trutta fario L.). The earliest calretinin-immunoreactive (CR-ir) cells were detected in the olfactory placode of 5-mm embryos.
Cecilia Thors et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 51(10), 1367-1373 (2003-09-23)
KLH (Keyhole limpet hemocyanin) is highly immunogenic, and crossreactive epitopes occur widely in nature. In schistosomiasis, infected hosts generate antibodies reactive with KLH. This is of diagnostic importance but we lack detailed information on the immunogen-carrying molecules and their distribution
Serglycin in quiescent and proliferating primary endothelial cells
Reine TM, et al.
Testing, 10(12), e0145584-e0145584 (2015)
C Thors et al.
Parasite immunology, 20(10), 489-496 (1998-11-03)
The number of individuals catching schistosomiasis has increased with the popularity of 'primitive tourism' in Africa. Highly immunogenic material originating from the intestine of intravascular adult schistosomes gives rise to an antibody response making possible early identification of infected individuals
Janeth J Peña et al.
PloS one, 11(12), e0168665-e0168665 (2016-12-31)
The parasitic flatworm Schistosoma mansoni, causative agent of human intestinal schistosomiasis in South America, relies importantly on the freshwater snail Biomphalaria glabrata as intermediate host to achieve development of cercariae that infect humans. The recommendation from the World Health Organization
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