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Safety Information

G9654

Sigma-Aldrich

Gentamicin-Glutamine

solution, suitable for cell culture, BioReagent

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.76

Product Name

Gentamicin-Glutamine solution, with 200 mM L-glutamine and 5 mg gentamicin per mL in tissue culture grade water, liquid, 0.1 μm filtered, BioReagent, suitable for cell culture

Quality Level

300

sterility

0.1 μm filtered

product line

BioReagent

form

liquid

potency

5-6 mg per mL

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria
mycoplasma

Mode of action

protein synthesis | interferes

shipped in

dry ice

storage temp.

−20°C

General description

Chemical structure: aminoglycoside

Application

Gentamicn-Glutamine solution is used for selection of transformed cells that contain the aacA.aphD gene, and is recommended for use in cell culture applications at 10 mL/L.

Biochem/physiol Actions

Mode of Action: The product inhibits protein synthesis by binding to L6 protein of the 50S ribosomal subunit. Glutamine functions as an essential as an energy source for most mammalian cells in culture because of its amino acid carbon skeletons.

Antimicrobial spectrum: This product acts against both gram-negative and gram-positive bacteria, and mycoplasma.

Components

This solution is formulated to contain 200 mM L-glutamine and 5 mg/mL gentamicin in .9% sodium chloride

Caution

This product is stable in solution at 37°C for 5 days. The recommended storage temperature is -20°C.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

G9654-BULK:
G9654-VAR:
G9654-10X5ML-PW:
G9654-10X5ML:

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Certificates of Analysis (COA)

Lot/Batch Number
098M4893V
028M4754V
106M4782V
075M4803V
074M4819V

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Articles

L-Glutamine in Cell Culture

Importance and uses of glutamine in hybridoma and mammalian cell culture

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