G9654

Gentamicin-Glutamine solution

with 200 mM L-glutamine and 5 mg gentamicin per mL in tissue culture grade water, liquid, 0.1 μm filtered, BioReagent, suitable for cell culture

• UNSPSC Code: 12352207

Properties

• Quality Level: 300
• sterility: 0.1 μm filtered
• product line: BioReagent
• form: liquid
• potency: 5-6 mg per mL
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• antibiotic activity spectrum: Gram-negative bacteria; Gram-positive bacteria; mycoplasma
• Mode of action: protein synthesis | interferes
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Chemical structure: aminoglycoside

Application

Gentamicn-Glutamine solution is used for selection of transformed cells that contain the aacA.aphD gene, and is recommended for use in cell culture applications at 10 mL/L.

Biochem/physiol Actions

Mode of Action: The product inhibits protein synthesis by binding to L6 protein of the 50S ribosomal subunit. Glutamine functions as an essential as an energy source for most mammalian cells in culture because of its amino acid carbon skeletons.

Antimicrobial spectrum: This product acts against both gram-negative and gram-positive bacteria, and mycoplasma.

Components

This solution is formulated to contain 200 mM L-glutamine and 5 mg/mL gentamicin in .9% sodium chloride

Caution

This product is stable in solution at 37°C for 5 days. The recommended storage temperature is -20°C.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves