G9043
Anti-GRP78/BiP (ET-21) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-78-kDa Glucose-Regulating Protein, Anti-Immunoglobulin Heavy Chain Binding Protein
About This Item
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biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 78 kDa
species reactivity
mouse, chicken, Xenopus, hamster, human, rat
enhanced validation
independent
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technique(s)
immunocytochemistry: 1:1,000 using methanol/acetone fixed cultured mouse fibroblast NIH3T3 cell line
immunohistochemistry: 1:1,000 using human cerebral cortex and human thyroid gland tissue sections
western blot: 1:3,000 using whole cell extract of human epitheloid carcinoma HeLa cell line
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... HSPA5(3309)
mouse ... Hspa5(14828)
rat ... Hspa5(25617)
Application
- for immunofluorescence staining of frozen heart tissue to examine whether ER stress signaling activation occurs in cardiomyocytes
- for immunohistochemistry on Paraffin embedded sections of human cerebral cortex and human thyroid gland tissue sections.
- to detect Bip by immunoblotting of protein extract from WT AB strain of zebrafish (Danio rerio)
- at a working dilution of 1:1000 to detect GRP78 in extract of prostate cancer cells after androgen deprivation therapy
Biochem/physiol Actions
Physical form
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
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JAN Code
G9043-200UL:
G9043-VAR:
G9043-BULK:
Certificates of Analysis (COA)
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