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G8132

Sigma-Aldrich

β-Glucuronidase from limpets (Patella vulgata)

Type L-II, lyophilized powder, 1,000,000-3,000,000 units/g solid

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:
9001-45-0
Enzyme Commission number:
3.2.1.31 (BRENDA, IUBMB)
EC Number:
232-606-8
MDL number:
MFCD00130629
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

limpet (Patella vulgata)

type

Type L-II

form

lyophilized powder

specific activity

1,000,000-3,000,000 units/g solid

shipped in

wet ice

storage temp.

−20°C

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Application

β-Glucuronidase Type L-II has been shown to be a superior enzyme for the hydrolysis of drug-glucuronides from urine.
New Technical Article Comparing Performance of Different Enzymes
Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes

Biochem/physiol Actions

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption. Morphine 3-β-D glucuronide was converted to morphine using ß-glucuronidase obtained from Patella vulgate.

Quality

Contains sulfatase activity which is inhibited by 0.1 M phosphate.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

G8132-500KU:
G8132-1MU:
G8132-2MU:
G8132-BULK:
G8132-VAR:
G8132-100KU:
G8132-5X1MU:
G8132-250KU:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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