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Safety Information

G7882

Sigma-Aldrich

L-Glutamic Dehydrogenase from bovine liver

Type III, lyophilized powder, ≥20 units/mg protein

Synonym(s):

L-GLDH, L-Glutamate:NAD[P]+ Oxidoreductase (deaminating), Glutamate Dehydrogenase from bovine liver

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About This Item

CAS Number:
9029-12-3
Enzyme Commission number:
1.4.1.3 (BRENDA, IUBMB)
EC Number:
232-848-4
MDL number:
MFCD00131461
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bovine liver

Quality Level

200

type

Type III

form

lyophilized powder

specific activity

≥20 units/mg protein

UniProt accession no.

P00366

storage temp.

−20°C

Gene Information

cow ... GLUD1(281785)

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Application

L-Glutamic Dehydrogenase was used to catalyzes the conversion of isocitrate into a-ketoglutarate and carbon dioxide.

Biochem/physiol Actions

Mammalian forms of this enzyme, including this bovine form, can use either NADP(H) or NAD(H) as coenzymes. L-glutamic dehydrogenase plays a unique role in mammalian metabolism. The reverse reaction catalyzed by this enzyme is the only pathway by which ammonia can become bound to the α-carbon atom of an α-carboxylic acid and thus, is the only source of de novo amino acid synthesis in mammalian species.

The bovine enzyme is characterized by three sets of properties:
  • It has a reversible concentration-dependent association, producing higher molecular weight forms.
  • Forms tight enzyme-reduced coenzyme-substrate ternary complexes whose rates of dissociation modulate the steady-state reaction rates.
  • Exhibits a wide variety of effects from the binding of any of a number of nucleotide modifiers.

L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.

Packaging

Package size based on protein content

Unit Definition

One unit will reduce 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.3 at 25 °C, in the presence of ammonium ions.

Physical form

Contains citrate and potassium phoshate buffer salts.

Analysis Note

Protein determined by biuret

substrate

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

G7882-10MG:
G7882-VAR:
G7882-25MG:
G7882-1G:
G7882-500MG:
QG7882-VAR:
G7882-50MG:
G7882-BULK:
QG7882-BULK:
G7882-100MG:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK9206
SLCH8002
SLCD5424
SLCB6959
SLBX7197

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