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G100120

Sigma-Aldrich

Sephadex® G-100

Medium

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About This Item

CAS Number:
9050-94-6
MDL number:
MFCD00132235
UNSPSC Code:
23151817
NACRES:
NA.56

Quality Level

100

form

slurry

technique(s)

LPLC: suitable

matrix

Agarose

matrix active group

polymer

swelling

1 g swells to 15-20 mL gel

bead size

40-120 μm

separation technique

size exclusion (SEC)

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General description

Sephadex® is composed of repeating glucose units attached by α-1,6 glucosidic bonds. This structure ensures more aptamer binding sites and greater binding capacity. It is suitable for large-scale purification and is considered stable and can be utilized more than once.
Fractionation Range (MW)
Globular Proteins: 4,000 - 150,000
Dextrans: 1,000 - 100,000

Application

Sephadex®G-100 is a gel filtration medium used in protein chromatography, affinity chromatography and gel filtration chromatography
It has been used:
  • in gel filtration column for α-amylase purification
  • in gel filtration column for the purification of polysaccharidic fraction extracted from the skin of the ray Raja radula
  • to remove excess protamine by size exclusion from cross-linked iron oxide (CLIO)-protamine-linked (B) peptides

Other Notes

G100120-100G′s updated product number is GE17-0060-01

Legal Information

Sephadex is a registered trademark of Cytiva

replaced by

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

G100120-50G:
G100120-100G:
G100120-BULK:
G100120-10G:
G100120-500G:
G100120-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Fat metabolism in higher plants. XLIV. Fatty acid synthesis by a soluble fatty acid synthetase from Sclanum tuberosum.
K P Huang et al.
Archives of biochemistry and biophysics, 143(2), 412-427 (1971-04-01)
R H Coleman et al.
Current microbiology, 32(3), 147-150 (1996-03-01)
Pseudomonas syringae isolate BR2R produces tabtoxin, a beta-lactam-containing antibiotic, and the causative agent of wildfire disease of green bean (Phaseolus vulgaris). beta-Lactamase production has been suggested as the mechanism that protects P. syringae from tabtoxin. We sought to determine whether
Production, purification and characterization of an a-amylase produced by <I>Saccharomycopsis fibuligera</I>
Gogoi, B.K., et al.
Journal of Applied Microbiology, 63(5), 373-379 (1987)
Gene sequence, modeling, and enzymatic characterization of alpha-amylase AmyLa from the thermophile Laceyella sp. DS3
El-Sayed AKA, et al.
Starch, 69(5-6), 1600255-1600255 (2017)
Sephadex-binding RNA ligands: rapid affinity purification of RNA from complex RNA mixtures
Srisawat C, et al.
Nucleic Acids Research, 29(2), e4-e4 (2001)
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