FA20

Fructose Assay Kit

sufficient for 20 assays

• Synonym(s): Fructose Test Kit
• EC Number: 200-333-3
• UNSPSC Code: 12164500
• NACRES: NA.84

Properties

• usage: sufficient for 20 assays
• Quality Level: 100
• analyte chemical class(es): sugars (fructose)
• technique(s): photometry: suitable
• application(s): food and beverages; general analytical
• storage temp.: 2-8°C

Description

Application

This kit is for the quantitative, enzymatic determination of fructose in food and other materials.Fructose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Fructose 6-phosphate is converted to glucose 6-phosphate by phosphoglucose isomerase (PGI). Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in the reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to fructose concentration.

Kit Components Only

• fructose standard 500 mg
• glucose assay reagent 1 mL/vial
• phosphoglucose isomerase 1 mL/vial

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3