F8648

Monoclonal Anti-Interferon-γ−Carboxyfluorescein antibody produced in mouse

clone 25723.11, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Monoclonal Anti-Interferon-γ
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: carboxyfluorescein conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 25723.11, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): flow cytometry: suitable
• isotype: IgG2b
• UniProt accession no.: P01579
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: human ... IFNG(3458)

Description

General description

Interferons (IFNs) are cytokines that are secreted in response to viral or bacterial infections and tumorigenesis. There are two types of IFNs, type I (IFN-α and IFN-β) and type II (IFN-γ). IFN-γ binds to specific receptor complex consisting of IFNγR1 and IFNγR2. There are many pathways that are mediated by IFN-γ binding such as JAK/STAT1, AP-1, NF-κB, STAT3 and STAT5. IFN-γ is pleotropic and performs various functions related to immune response, inflammation, differentiation and activation of T cells, cell cycle and apoptosis. The most studied function of IFN-γ is priming the antigen presenting cells by up regulating major histocompatibility (MHC) Class I molecules. IFN-γ has clinical applications in autoimmune diseases (rheumatoid arthritis), multiple sclerosis, cancer, HIV and fungal infections. Anti-Interferon-γ recognizes intracellular human IFN-γ. The product is prepared by conjugation of carboxyfluorescein to Protein G purified IFN-γ monoclonal antibody.

Specificity

The conjugates will identify and quantitate cells containing intracellular forms of human IFN-γ by flow cytometry.

Immunogen

recombinant human IFN-γ

Application

Anti-interferon-γ antibody may be used for detection of intracellular IGN-γ in peripheral blood lymphocytes by adding 10 μL of the antibody to 1-5 X 10⁵ cells.

Physical form

Solution in phosphate buffered saline containing 0.1% sodium azide.

Preparation Note

Purified antibody is conjugated to carboxyfluorescein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves