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Safety Information

F7253

Sigma-Aldrich

Fast Black K Salt hemi(zinc chloride) salt

Dye content, ≥25%, practical grade, powder

Synonym(s):

2,5-Dimethoxy-4-([4-nitrophenyl]azo)benzenediazonium chloride hemi(zinc chloride) salt, Brentamine fast black K salt

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About This Item

Linear Formula:
C14H12N5O4 · 1/2ZnCl4
CAS Number:
Molecular Weight:
417.88
Colour Index Number:
37190
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Fast Black K Salt hemi(zinc chloride) salt, practical grade

grade

practical grade

Quality Level

form

powder

composition

Dye content, ≥25%

technique(s)

microbe id | staining: suitable

color

brown-red

mp

150 °C

solubility

water: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc([N+]#N)c(OC)cc1N=Nc2ccc(cc2)[N+]([O-])=O.COc3cc([N+]#N)c(OC)cc3N=Nc4ccc(cc4)[N+]([O-])=O

InChI

1S/2C14H12N5O4.4ClH.Zn/c2*1-22-13-8-12(14(23-2)7-11(13)16-15)18-17-9-3-5-10(6-4-9)19(20)21;;;;;/h2*3-8H,1-2H3;4*1H;/q2*+1;;;;;+2/p-4

InChI key

NZWZSUSPTZUGNN-UHFFFAOYSA-J

General description

Fast black K (C.l. 37190) is a lipophilic amino-substituted monoazo dye. It is also known as Brentamine fast black K salt.

Application

Fast Black K Salt hemi (zinc chloride) salt has been used for the staining of acid phosphatase activity in SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and native-PAGE. It has been used for staining endopeptidase and leucine aminopeptidase activity.

Fast black K salt has various applications as listed below-

  • to stain enterochromaffin cells > as an indirect stain for periodate-generated tissue aldehyde groups
  • as a visualization agent for amphetamines and cyanobacterial toxins following their separation by thin-layer chromatography
  • for the staining of acid phosphatase activity in SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and native-PAGE
  • staining endopeptidase and leucine aminopeptidase activity

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PRTR

Class I Designated Chemical Substances

JAN Code

F7253-VAR:
F7253-BULK:
F7253-25G:
F7253-100G:
F7253-50G:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Analyses of Root-secreted Acid Phosphatase Activity in Arabidopsis.
Wang L and Liu D
Bio-protocol null
Genetic control of isozymes in the primary gene pool Phaseolus lunatus L.
Zoro BI
Biotechnologie, agronomie, societe et environnement, 3, 10-27 (1999)

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