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Safety Information

F5305

Sigma-Aldrich

Monoclonal Anti-Fibroblast Growth Factor-Basic antibody produced in mouse

clone 10060, purified immunoglobulin, lyophilized powder

Synonym(s):

Anti-FGF2

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About This Item

MDL number:
UNSPSC Code:
51111800
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

10060, monoclonal

form

lyophilized powder

species reactivity

human

technique(s)

capture ELISA: 2-8 μg/mL

isotype

IgG2a

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... FGF2(2247)

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General description

Fibroblast Growth Factor Basic (also known as bFGF) is a pleotropic cytokine that that is a potent mitogenic factor. On binding to its cognate receptor tyrosine kinase, FGFR, bFGF is capable of activation of pathways such as MAPK and PI3K and produce cellular effects in varied cell types. Fibroblast Growth Factor-basic is repoted to induce proliferation of fibroblasts, differentiation, wound healing and regeneration, limb formation, tissue remodelling and angiogenesis. The role of bFGF in chondrocyte proliferation and repair has established the role of bFGF in maintaining cartilage and intervertebral disc homeostasis. Along with VEGF, bFGF is important in angiogenesis and tumorigenesis in response to inflammatory cytokines
Monoclonal Anti-Fibroblast Growth Factor Basic recognizes human Fibroblast Growth Factor Basic. It also recognizes bovine bFGF. It shows 0.2% cross-reactivity with bovine FGF acidic. It does not react with human FGF acidic, FGF-4, -6 or -7.

Immunogen

purified, E. coli-derived recombinant human fibroblast growth factor basic.

Application

Monoclonal Anti-Fibroblast Growth Factor Basic may be used as capture antibody in ELISA at a recommended concentration range of 2-8 μg/ml.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

F5305-BULK:
F5305-VAR:
F5305-500UG-PW:
F5305-500UG:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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H Schmal et al.
Cytotherapy, 9(2), 184-193 (2007-04-25)
The possible functional role of basic fibroblast growth factor (bFGF) in regulating the mitotic and metabolic activity of primary human articular chondrocytes was investigated. [EF1]Chondrocytes were enzymatically isolated from femoral head cartilage, and were cultured in vitro in monolayer. bFGF-dependent
Shintaro Kanayama et al.
Experimental eye research, 85(6), 772-781 (2007-10-02)
The aim of this study was to compare angiogenesis-induction capabilities of cultured corneal epithelial cells (CCE) and cultured oral mucosal epithelial cells (COE) in vitro, and identify candidate factors that induce corneal neovascularization after transplantation of COE sheets. Rabbit corneal
Michael B Ellman et al.
Gene, 420(1), 82-89 (2008-06-21)
Two members of the fibroblast growth factor (FGF) family, basic FGF (bFGF) and FGF-18, have been implicated in the regulation of articular and intervertebral disc (IVD) cartilage homeostasis. Studies on bFGF from a variety of species have yielded contradictory results
Harris Pratsinis et al.
European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society, 16(11), 1858-1866 (2007-09-04)
Intervertebral disc (IVD) degeneration is frequently characterized by increased cell proliferation, probably as a tissue regenerative response. Although many growth factors and their receptors have been shown to be expressed normally in the disc, and generally to be over-expressed during
Maria Bodo et al.
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, 22(6), 621-630 (2002-08-07)
The Crouzon syndrome, which is associated with fibroblast growth factor receptor (FGFR2) mutations, is characterized by premature fusion of cranial sutures. We used an in vitro model of cultured periosteal fibroblasts from normal subjects and from Crouzon patients with FGFR2

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