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E7029

Sigma-Aldrich

pBICEP-CMV™-3 Expression Vector

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About This Item

UNSPSC Code:
12171500
NACRES:
NA.56

shipped in

dry ice

Quality Level

200

storage temp.

−20°C

General description

The pBICEP-CMV-3 bicistronic expression vector is a 5.3 kb derivative of pCMV5 used for transient or stable co-expression of an N-terminal 1´ FLAGÒ fusion protein and a selection gene in mammalian cells.

Application

For transient, cytoplasmic expression of an N-terminal FLAG® fusion and a second gene of interest or selection marker from bicistronic mRNA. Two genes can be cloned into MCS1 and MCS2 for transcription of a single message from the CMV promoter. This vector is useful for protein-protein interaction studies, multi-subunit proteins, and cloning a selection marker of choice.

Biochem/physiol Actions

The presence of two multiple cloning sites not only allows the user to clone a target gene, but a selectable marker of their choice, as well. The promoter-regulatory region of the human cytomegalovirus immediate early promoter drives transcription of the FLAG-fusion construct along with a downstream selection gene. The EMCV IRES region controls translation of the selection gene by recruiting the ribosomal subunits for cap-independent translational initiation. Depending on the mode of selection, stable transfectants can be generated by transfection using the appropriate ESCORTä product line specific for the cell type.

Other Notes

Browse additional application references in our FLAG® Literature portal.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pBICEP-CMV is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

E7029-BULK:
E7029-VAR:
E7029-BULK-N:
E7029-20UG:

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