Skip to Content
Merck
All Photos(1)

Documents

Safety Information

E0412

Sigma-Aldrich

Carboxylesterase 2 human

recombinant, expressed in baculovirus infected BTI insect cells

Synonym(s):

Carboxylesterase 2 human, CES2, CES2A1

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Enzyme Commission number:
3.1.1.1 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in baculovirus infected BTI insect cells

Quality Level

200

form

liquid

specific activity

≥500 units/mg protein




mol wt

60 kDa

weight

(0.5 ml)

shipped in

dry ice

storage temp.

−70°C

Looking for similar products? Visit Product Comparison Guide

General description

Carboxylesterase 2 human gene is mapped to human chromosome 16q22.1.
Carboxylesterase belongs to the α/β hydrolase fold family and is involved in drug metabolism and activation. It is present in colon, liver, small intestine, heart, brain and testis. The hydrolase activity of small intestine is attributed to carboxylesterase 2 human. It has two glycosylation sites at 103 and 267 residues with molecular mass 60kDa.

Application

Carboxylesterase 2 human has been used:
  • in the in vitro enzyme-based inhibitor screening assay
  • in substrate selectivity assay with emission ratiometric two-photon probe (SE1)
  • as a reference standard in the enzyme assay with various 4-nitrophenyl and 1-naphthyl based substrates

Carboxylesterase 2 may play a key role in biotransformation of a variety of ester containing drugs and prodrugs.
Delivers high catalytic activity, ideal for robust high-throughput screening assays including drug-drug interaction studies, and pharmacokinetic studies for evaluating pro-drugs and non-CYP pathways of elimination.

Biochem/physiol Actions

Carboxylesterase 2 human identifies the site containing a large alcohol and small acyl group. It shows less transesterification activity due to presence of conformational interference in active site.
Member of a serine esterase family that hydrolyze ester and amide bonds. Carboxylesterase 2 is an endoplasmic reticulum-bound hydrolase that plays a critical role in xenobiotic detoxification and activation for ester-containing therapeutics. Carboxylesterase 2 is also involved in the detoxification of drugs such as heroin and cocaine. This enzyme is thought to play a role in lipid metabolism.

Unit Definition

One unit will produce 1 nanomole of 4-nitrophenol from 4-nitrophenyl acetate per minute at pH 7.4 at 37 deg C.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Cartagena Act

Cartagena Act Listed

JAN Code

E0412-VAR:
E0412-1VL:
E0412-1VL-PW:
E0412-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Human liver carboxylesterase. Purification and molecular properties.
W Junge et al.
Archives of biochemistry and biophysics, 165(2), 749-763 (1974-12-01)
T Satoh et al.
Annual review of pharmacology and toxicology, 38, 257-288 (1998-05-23)
Multiple carboxylesterases (EC 3.1.1.1) play an important role in the hydrolytic biotransformation of a vast number of structurally diverse drugs. These enzymes are major determinants of the pharmacokinetic behavior of most therapeutic agents containing ester or amide bonds. Carboxylesterase activity
Tetsuo Satoh et al.
Drug metabolism and disposition: the biological fate of chemicals, 30(5), 488-493 (2002-04-16)
This article reports on a symposium sponsored by the American Society for Pharmacology and Experimental Therapeutics and held at the April 2001 Experimental Biology meeting. Current developments in molecular-based studies into the structure and function of cholinesterases, carboxylesterases, and paraoxonases
Teruko Imai
Drug metabolism and pharmacokinetics, 21(3), 173-185 (2006-07-22)
Human carboxylesterase 1 (hCE-1, CES1A1, HU1) and carboxylesterase 2 (hCE-2, hiCE, HU3) are a serine esterase involved in both drug metabolism and activation. Although both hCE-1 and hCE-2 are present in several organs, the hydrolase activity of liver and small
Jianqin Zhang et al.
Chemosphere, 93(6), 1207-1215 (2013-08-01)
Carboxylesterases (CarEs) play key roles in metabolism of specific hormones and detoxification of dietary and environmental xenobiotics in insects. We sequenced and characterized CarE cDNAs putatively derived from two different genes named LmCesA1 and LmCesA2 from the migratory locust, Locusta
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service