DUO96040
Duolink® PLA Multicolor Probemaker Kit - Far Red
Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events
Synonym(s):
in situ Proximity Ligation Assay, Flowcytometry-PLA, Protein Protein Interaction Kit
About This Item
Recommended Products
technique(s)
immunofluorescence: suitable
multiplexing: suitable
proximity ligation assay: suitable
fluorescence
λex 646 nm; λem 664 nm (Far Red)
λex 646 nm; λem 664 nm
suitability
suitable for fluorescence
shipped in
dry ice
storage temp.
−20°C
Application
Far Red fluorescence detection reagents are often used with Cy®5 filter.
Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
Features and Benefits
- Detect multiple PPI, PTM and low abundant protein events in a single sample.
- Visualize protein complex formation and dissociation
- Monitor protein signaling pathways
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Detection of low abundant proteins due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Faster and more simple than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Components
Duolink® PLA Multicolor Probemaker Kit - Far Red contains all the necessary reagents to perform the conjugations of Far Red Oligo H and Far Red Oligo I directly to primary antibodies. The resulting PLA probes (Far Red H and Far Red I) must be used together and cannot be paired with any other Duolink® PLA probe.
This product is comprised of the following:
- Far Red Oligo H DUO86040H
- Far Red Oligo I DUO86040I
- Conjugation Buffer DUO82033
- Stop Reagent DUO82034
- Storage Solution DUO82035
See datasheet for more information.
To perform a Multicolor Duolink® PLA experiment primary antibodies (PLA, IHC, ICC or IF validated) that recognize protein targets of interest are required. Duolink® PLA Multicolor Probemaker-generated probes (Red A/Red B, Green C/Green D, Orange F/Orange G, and/or FarRed H/FarRed I) are used in combination to perform the Multiplex experiment.The reagents in the Duolink® PLA Multicolor Reagent Pack are required to perform the amplification and detection of the resulting PLA probes. Required additional reagents include Duolink® Wash Buffers and Mounting Medium.
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
PDSCL
Please refer to KIT Component information
PRTR
Please refer to KIT Component information
FSL
Please refer to KIT Component information
ISHL Indicated Name
Please refer to KIT Component information
ISHL Notified Names
Please refer to KIT Component information
Cartagena Act
Please refer to KIT Component information
JAN Code
キットコンポーネントの情報を参照してください
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Articles
Duolink® PLA Multicolor technology and the quantification of protein-protein interactions in fixed tissues and cells.
Protocols
Duolink® PLA Multicolor Detection Protocol
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service