Skip to Content
Merck
All Photos(1)

Key Documents

Safety Information

D8406

Sigma-Aldrich

Anti -Dinitrophenyl antibody, Mouse monoclonal

IgE isotype, ~1 mg/mL, clone SPE-7, affinity purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-Dinitrophenyl, Anti Dnp Ige, Anti-DNP IGE - Monoclonal Anti-Dinitrophenyl antibody produced in mouse, Anti-Dnp Ige, Monoclonal Anti-DNP

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

SPE-7, monoclonal

form

buffered aqueous solution

concentration

~1 mg/mL

technique(s)

indirect ELISA: 0.05-0.1 μg/mL using using 0.5 μg/well DNP-BSA as the coating antigen and 1 μg/mL Monoclonal Anti-Mouse IgE, Peroxidase conjugate as the detector antibody.

isotype

IgE

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Looking for similar products? Visit Product Comparison Guide

General description

Dinitrophenyl (DNP) is a hapten that is often used for labeling primary or secondary probes in immunological assays. Hence, anti-DNP antibody is a useful tool for the detection and analysis of target molecules. Monoclonal Anti-DNP antibody is specific for DNP.
IgE gene is encoded on human chromosome 14. IgE is the least circulating antibody, but critical for eliciting hypersensitivity reaction against allergens.

Immunogen

DNP-KLH conjugate

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Monoclonal Anti-Dinitrophenyl antibody is suitable for use in flow cytometry and immunocytochemistry.
Monoclonal antidinitrophenyl specific mouse IgE has been used in degranulation of rat mast cells using microplate reader and fluorescence spectrophotometer.
The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.
The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.To induce systemic anaphylaxis, mice were injected with 3 μg of anti-DNP mouse IgE and then given by IV 500 μg of DNP-BSA twenty hours later. To induce local anaphylaxis, mice were injected intradermally with 10ng of anti-DNP mouse IgE and then challenged with an i.v. injection of 500 μg DNP-BSA.

Biochem/physiol Actions

IgE is produced by B cells by class switch recombination in response to allergens. IgE is also associated with mast cells and is abundantly produced in response to allergens especially in asthma and allergic rhinitis. IgE regulates mast cell homeostasis and is upregulated in food allergy. IgE is implicated in the pathogenesis of autoimmune diseases like rheumatoid arthritis and systemic lupus erythematosus. IgE levels are modulated by other genes present on chromosome 14 especially in patients with asthma. Patients with mutations in signal transducer and activator of transcription 3 (STAT3) are associated high levels of IgE called the hyper–immunoglobulin E syndrome (HIES).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

D8406-.2MG:
D8406-BULK:
D8406-VAR:
D8406-.5MG:
D8406-100UG:
IXO67685:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Ae-Yeon Lee et al.
Biomolecules & therapeutics, 24(3), 283-290 (2016-05-03)
Oroxylum indicum has long been used in Asian traditional medicine to prevent and treat respiratory diseases, diabetes, diarrhea and other conditions. Oroxylin A is a flavone that is present in Oroxylum indicum and in Scutellaria baicalensis. Because the root extracts
Nathalie Launay et al.
Acta neuropathologica, 129(3), 399-415 (2015-01-01)
X-linked adrenoleukodystrophy (X-ALD) is a rare neurometabolic disease characterized by the accumulation of very long chain fatty acids (VLCFAs) due to a loss of function of the peroxisomal transporter ABCD1. Here, using in vivo and in vitro models, we demonstrate
Immunoglobulin E.
William EW, et al.
Archives of Pathology & Laboratory Medicine, 124(9), 1382-1385 (2000)
A processed human immunoglobulin epsilon gene has moved to chromosome 9.
Battey J, et al.
Proceedings of the National Academy of Sciences of the USA, 79(19), 5956-5960 (1982)
The who, where, and when of IgE in allergic airway disease.
Dullaers M, et al.
The Journal of Allergy and Clinical Immunology, 129(3), 5024-5032 (2012)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service