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Safety Information

D4764

Sigma-Aldrich

Deoxyribonucleic acid from calf thymus

Genomic, unsheared

Synonym(s):

double-stranded template DNA, Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid

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About This Item

CAS Number:
91080-16-9
EC Number:
293-507-3
MDL number:
MFCD00151698
UNSPSC Code:
41106310
eCl@ss:
32160414
NACRES:
NA.52

grade

for molecular biology

form

lyophilized powder

mol wt

>20 kb

storage temp.

2-8°C

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General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Specificity

42% GC; Tm = 87 °C.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay. It was used as reference to determine the DNA content in cartilage tissue digest samples.

Features and Benefits

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Preparation Note

Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of ~2 A260 units per mL. ~20 A260 units per mg DNA.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

D4764-VAR:
D4764-1UN:
D4764PROC:
D4764-BULK:
D4764-.25UN:
D4764-5UN:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCG1123
SLCC6539
SLBZ0092
SLCB5336
SLBX1835

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