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D3287

Sigma-Aldrich

Deoxyribonucleic acid, single stranded from human placenta

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352200

grade

for molecular biology

description

For hybridization

form

solution

mol wt

(Fragments from 587-831 bp.)

solubility

water: 9-12 mg/mL

storage temp.

−20°C

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

InChI key

AWBASQCACWFTGD-UHFFFAOYSA-N

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General description

Human placental DNA is isolated from donor placenta, but will contain some maternal DNA. The DNA fragments are sonicated to produce fragments of consistent size.

Application

Sonicated Deoxyribonucleic acid, single stranded from human placenta, was used as blocking agent in Southern hybridization of DNA from human papillomavirus (HPV) positive SiHa, HeLa and CaSki cell-lines. It was used as standard in GC/MS analysis of exocyclic DNA adducts.
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.

In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.

Features and Benefits

• High quality human DNA.
• DNA fragments of defined sizes.

Components

DNA is supplied in a solution of 100mM phosphate buffer.  This is a ready to use concentrated solution
of 9-12 mg/ml DNA in 100mM phosphate buffer. However, it will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use. Cooling on ice will
reduce the chances for reannealing, as it is more likely to reanneal if cooled at room temperature.

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Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

D3287-5X1ML:
D3287-VAR:
D3287-1ML:
D3287-1ML-PW:
D3287-BULK:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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H J Chen et al.
Chemical research in toxicology, 12(12), 1119-1126 (1999-12-22)
Exocyclic DNA adducts have been reported to derive from various exogenous as well as endogenous sources, such as lipid peroxidation. Among them, 1,N(6)-ethenoadenine (epsilonAde) has previously been detected in tissue DNA of untreated rodents and humans by an immunoaffinity/(32)P-postlabeling method.
Mark F Evans et al.
BMC clinical pathology, 3(1), 2-2 (2003-06-13)
BACKGROUND: Over the past five years in situ hybridization techniques employing tyramide amplification reagents have been developed and promise the potential detection of low/single-copy nucleic acid sequences. However the increased sensitivity that tyramide amplification brings about may also lead to
M Iu Mazina et al.
Tsitologiia, 55(4), 218-224 (2013-07-24)
DNA replication begins from multiple sites distributed thoughout the genome and named replication origins. Despite the increasing amount of data on the properties of replication origins, it is still unknown what factors(s) is the primary determinant of ORC localization. Su(Hw)
Non-invasive prenatal aneuploidy testing: technologies and clinical implication.
Brynn Levy et al.
MLO: medical laboratory observer, 45(6), 8-8 (2013-07-24)
Christine Keyser et al.
Medecine sciences : M/S, 29(6-7), 637-641 (2013-07-19)
The authors highlight the opportunities to reconstruct the human Eurasian steppe migration movements with the analyses of nuclear DNA markers (short tandem repeats on autosomal DNA and on the Y chromosome) as well as mitochondrial DNA markers. They studied 26 ancient

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