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Safety Information

D2197

Sigma-Aldrich

Diaphorase from Clostridium kluyveri

recombinant, expressed in E. coli, Animal-component free

Synonym(s):

Diaphorase, Lipoamide Dehydrogenase, Lipoyl Dehydrogenase

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About This Item

Enzyme Commission number:
1.8.1.4 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

200

form

solid

specific activity

>=30 units/mg protein (biuret)

storage temp.

−20°C

General description

Research area: Cancer

Application

Diaphorase from Clostridium kluyveri has been used:
  • in lactate dehydrogenase assay in gill cells.
  • impregnated on the strip for formate dehydrogenase assay.
  • in various assays to oxidate β-NADH or β-NADPH in the presence of an electron acceptor.
  • for semiquinone generation during benzoquinone reduction.
  • for evaluation of glucose uptake in cultured muscle cells.
  • in lactate dehydrogenase cytotoxicity assay.

Biochem/physiol Actions

Diaphorase catalyzes the oxidation of either β-NADH or β-NADPH in the presence of an electron acceptor such as methylene blue or 2,6-dichlorophenolindophenol. Diaphorases that are specific for either β-NADH or β-NADPH are known. The pig heart enzyme of Straub seems to have native diaphorase (β-NADH specific) as well as lipoic and lipoamide dehydrogenase activities.

Unit Definition

One unit of diaphorase will oxidize 1.0 micromole of beta-NADH per minute at pH 7.5 at 25 degrees C, with the corresponding reduction of the appropriate electron acceptor.

Physical form

Supplied as a lyophilized powder containing trehalose.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

D2197-100UN-PW:
D2197-300UN-PW:
D2197-500UN-PW:
D2197-VAR:
D2197-500UN:
D2197-BULK:
D2197-300UN:
D2197-100UN:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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