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D1667

Sigma-Aldrich

Monoclonal Anti-Dynein (Heavy Chain) antibody produced in mouse

clone 440.4, ascites fluid

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About This Item

MDL number:
MFCD00164585
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

440.4, monoclonal

mol wt

antigen 440 kDa

contains

15 mM sodium azide

species reactivity

human, chicken

technique(s)

indirect ELISA: suitable
western blot: 1:100 using a chick brain extract or Kinesin enriched rat brain extract

isotype

IgG2a

UniProt accession no.

Q8TD57

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DNAH1(25981) , DNAH2(146754) , DNAH3(55567) , DNAH5(1767)

General description

Monoclonal Anti-Dynein (Heavy Chain) (mouse IgG2a isotype) is derived from the 440.4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Dynein axonemal heavy chain 1 (DNAH1) encodes for an inner dynein heavy chain. This gene is mapped to the human chromosome location 3p21.1. DNAH1 is expressed in the testis and other ciliated cells. Dyneins are composed of two or three distinct heavy chains, three intermediate chains and at least four light chains.

Immunogen

cytoplasmic dynein from chicken embryo brain.

Application

Monoclonal Anti-Dynein (Heavy Chain) antibody produced in mouse has been used in:
  • indirect immunofluorescence at 1:50 dilution
  • immunocytochemistry
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • dot blot

Biochem/physiol Actions

Dynein has also been implicated in cytoplasmic motile functions, including chromosomal movement, retrograde organelle and axonal transport. It also regulates the endocytic pathway and the organization of the Golgi apparatus. Mutations in dynein axonemal heavy chain 1 (DNAH1) are associated with primary ciliary dyskinesia and multiple morphological anomalies of the flagella (MMAF) that leads to asthenozoospermia and male infertility.
Dynein is a motor protein that regulates the movement of organelles to the ‘minus′ end of microtubules. These motor proteins are also involved in spindle organization, retrograde transport and chromosome movement

Physical form

Suppied as ascites fluid with 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

D1667-.5ML:
D1667-VAR:
D1667-.2ML:
D1667-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mariem Ben Khelifa et al.
American journal of human genetics, 94(1), 95-104 (2013-12-24)
Ten to fifteen percent of couples are confronted with infertility and a male factor is involved in approximately half the cases. A genetic etiology is likely in most cases yet only few genes have been formally correlated with male infertility.
R E Fowler et al.
Molecular and biochemical parasitology, 117(2), 187-200 (2001-10-19)
We have studied the occurrence, stage specificity and cellular location of key molecules associated with microtubules in Plasmodium falciparum merozoites. Antibodies to gamma tubulin, conventional kinesin and cytoplasmic dynein were used to determine the polarity of merozoite microtubules (mt), the
L Wordeman et al.
The Journal of cell biology, 114(2), 285-294 (1991-07-01)
We have used indirect immunofluorescence in combination with correlative EM to subdivide the mammalian kinetochore into two domains based on the localization of specific antigens. We demonstrate here that the fibrous corona on the distal face of the kinetochore plate
Jason Yi et al.
The Journal of cell biology, 202(5), 779-792 (2013-08-28)
T cells rapidly reposition their centrosome to the center of the immunological synapse (IS) to drive polarized secretion in the direction of the bound target cell. Using an optical trap for spatial and temporal control over target presentation, we show
K T Vaughan et al.
The Journal of cell biology, 131(6 Pt 1), 1507-1516 (1995-12-01)
Cytoplasmic dynein is a retrograde microtubule motor thought to participate in organelle transport and some aspects of minus end-directed chromosome movement. The mechanism of binding to organelles and kinetochores is unknown. Based on homology with the Chlamydomonas flagellar outer arm
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