CS0260

Glutathione Assay Kit

sufficient for 700 assays

• Synonym(s): GSH Quantification Test
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 700 assays
• Quality Level: 200
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: human ... GSR(2936)

Description

General description

The kit provides all reagents for simple and quick assay to measure the level of total glutathione (GSSG + GSH) in a cell and tissue extracts and in red blood cells or plasma. The sample is first deproteinized with the 5% 5-sulfosalicylic acid solution. Glutathione content of the sample is then assayed using a kinetic assay in which catalytic amounts of glutathione cause a continuous reduction of 5,5′-dithiobis-(2-nitrobenzoic) acid (DTNB) to TNB. The oxidised glutathione formed is recycled by glutathione reductase and NADPH. The product, TNB, is assayed colorimetrically at 412 nm.

Application

Glutathione Assay Kit has been used to measure total glutathione concentration in glutathione assay. It also has been used to determine different oxidative stress parameters in rats.

Biochem/physiol Actions

Glutathione (GSH) functions as a cofactor for several antioxidant enzymes. It is involved in the regeneration of vitamins C and E. It modulates cellular proliferation and apoptosis. GSH is crucial for mitochondrial functioning and the maintenance of mitochondrial DNA (mtDNA). Mutations in this gene are associated with Alzheimer′s, Parkinson′s and Huntington′s diseases. It is also linked to cystic fibrosis, immune diseases and cardiovascular diseases.

Reduced glutathione (GSH), a tripeptide (g-glutamyl-cysteinylglycine), is the major free thiol in most living cells and is involved in many biological processes such as detoxification of xenobiotics, removal of hydroperoxides, and maintenance of the oxidation state of protein sulfhydryls. It is the key antioxidant in animal tissues

Features and Benefits

• A quick and simple method
• The kit contains all the reagents required for a colorimetric assay of total glutathione (oxidised + reduced GSSG + GSH)
• The kit includes a deproteination reagent for biological samples

Kit Components Only

• Assay Buffer 5x 30 mL
• DTNB 8 mg
• DMSO 7.5 mL
• Glutathine Reductase 20 U
• L-Glutathione Reduced 300 μg
• NADPH 25 mg
• 5-Sulfosalicylic Acid 2.5 g

Safety Information

• Pictograms: GHS08,GHS05
• Signal Word: Danger
• Hazard Statements: H314,H334
• Precautionary Statements: P261 - P280 - P284 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• Flash Point(F): 188.6 °F
• Flash Point(C): 87 °C