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CLL1220

Sigma-Aldrich

Safe Harbor Landing Pad Cell Line A549 Cancer Cells

human male lung (Source Disease: Carcinoma)

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

Product Name

Safe Harbor Landing Pad Cell Line A549 Cancer Cells,

biological source

human male lung (Source Disease: Carcinoma)

Quality Level

200

form

frozen liquid (Vial of Frozen Cells)

growth mode

Adherent

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

General description

The STR profile of this cell line matches that of its parental cell line ATCC® Catalog No. CCL-185a. A549 is a human lung carcinoma cell line isolated in 1972 from a lung tumor of a male patient suffering from carcinoma.

Cell Line Description

These cells are a human lung carcinoma cell line isolated from a lung tumor of a 58-year old male suffering from carcinoma. The cells possess a hypotriploid karyotype and express EGFR and mutant KRAS.

Application

This product is a human A549 adenocarcinoma cell line in which a landing pad cassette has been integrated into the AAVS1 safe harbor locus using CompoZr®Zinc Finger Nuclease technology. The mKATE2 fluorescence gene was integrated following the EF1a promoter and flanked by unique Cre-lox sites. The design of this landing pad cassette allows for easy, fast, and affordable genetic modification using Cre recombinase. mKATE2 can easily be exchanged for a payload of the user′s choice using Cre recombinase and a targeting vector with appropriate lox sites. Cells can then be sorted via fluorescence-activated cell sorting (FACS) for loss of mKATE2 expression as a surrogate for successful integration of the targeting vector. Approximately 7-10 days are required for loss of the mKATE2 signal in successfully targeted cells. See technical bulletin for detailed protocols.

Features and Benefits

These cells contain the mKATE2 fluorescence gene flanked by unique Cre-lox sites inserted in the AAVS1 safe harbor gene. These A549 cells are adherent with a doubling time of approximately 22 hours.

Quality

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Culture Medium

RPMI modified to contain 2mM L-glutamine, 10mM HEPES, 1mM sodium pyruvate, and 1500mg/L sodium bicarbonate. Add 10% FBS(Catalog Number F2442).

Legal Information

ATCC is a registered trademark of American Type Culture Collection
CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

CLL1220A-1VL:
CLL1220-BULK:
CLL12205YR-1VL:
CLL1220-1VL:
CLL1220-VAR:
CLL1220AM-1VL:

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Certificates of Analysis (COA)

Lot/Batch Number
SLBZ2010

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Kimi Araki et al.
Nucleic acids research, 30(19), e103-e103 (2002-10-05)
The Cre-lox system is an important tool for genetic manipulation. To promote integrative reactions, two strategies using mutant lox sites have been developed. One is the left element/right element (LE/RE)-mutant strategy and the other is the cassette exchange strategy using
Huseyin Tas et al.
PloS one, 10(9), e0136963-e0136963 (2015-09-04)
We describe an optimized system for the easy, effective, and precise modification of the Escherichia coli genome. Genome changes are introduced first through the integration of a 1.3 kbp Landing Pad consisting of a gene conferring resistance to tetracycline (tetA)
Zhong-Wei Du et al.
Stem cells (Dayton, Ohio), 27(5), 1032-1041 (2009-05-06)
To circumvent the silencing effect of transgene expression in human embryonic stem cells (hESCs), we employed the Cre recombination-mediated cassette exchange strategy to target the silencing-resistant site in the genome. We have identified new loci that sustain transgene expression during

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