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biological source
bovine blood
growth mode
Suspension
karyotype
2n = 60, aneuploid, modal no. 65
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Bovine leukaemic lymphoblast
Cell Line Description
BL-3 was established from a neoplastic lymph node of a 3 month old male calf. BVDV positive.
Application
Virus studies
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 5-9x100,000 cells/ml; 5% CO2; 37°C. Cells should not be over diluted.
Other Notes
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Certificates of Analysis (COA)
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