A431 Cell Line human

85090402, squamous carcinoma

• Synonym(s): A-431 Cells, A431/P Cells
• UNSPSC Code: 41106514

Properties

• biological source: human skin
• growth mode: Adherent
• karyotype: Hypertriploid
• morphology: Epithelial
• products: Not specified
• receptors: Epidermal growth factor (EGF)
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human squamous carcinoma

Cell Line Description

Derived from an epidermal carcinoma of the vulva taken from an 85 yr old female. The cells carry large numbers of EGF binding sites and is an indicator cell for anti-TGF binding.

Application

A431 Cell Line human has been used:

• to demonstrate that p38 mitogen-activated protein kinase enhances epidermal growth factor-induced apoptosis in A431 cells
• to study melanoma-specific CD44 (a tumor marker and transmembrane receptor) alternative splicing pattern in A431 cells
• to study the anti-cancer activity of antioxidant-rich Cameroonian medicinal plants

Cell cycle studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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